Mercuric-Chloride-lnduced Autoimmunity in Mice Involves Up-Regulated Presentation by Spleen Cells of Altered and Unaltered Nucleolar Self Antigen
| Autor: | Peter Griem, Malgorzata Kubicka-Muranyi, Reinhard Lührmann, Birgit Lübben, Norbert Rottmann, Ernst Gleichmann |
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| Rok vydání: | 1995 |
| Předmět: |
CD4-Positive T-Lymphocytes
Chromosomal Proteins Non-Histone T cell Immunology Antigen presentation Autoimmunity Mice Inbred Strains Biology Autoantigens Epitope Autoimmune Diseases Histones Mice Antigen medicine Animals Immunology and Allergy Fibrillarin Antigen Presentation Hybridomas Histocompatibility Antigens Class II General Medicine T lymphocyte Clone Cells Up-Regulation medicine.anatomical_structure Ribonucleoproteins Mercuric Chloride Female Clone (B-cell biology) Spleen Small nuclear ribonucleoprotein Protein Binding |
| Zdroj: | International Archives of Allergy and Immunology. 108:1-10 |
| ISSN: | 1423-0097 1018-2438 |
| DOI: | 10.1159/000237110 |
| Popis: | HgCl2 treatment of B10.S mice induces IgG autoantibodies to fibrillarin, a component of small nuclear ribonucleoprotein particles, and histone. Here, we demonstrate the activation by HgCl2 of autoreactive T cells specific for these nuclear proteins. Of nine CD4+ T cell hybridoma clones obtained from HgCl2-treated B10.S mice, one clone reacted to histone Hl and eight clones to fibrillarin. One of the fibrillarin-specific clones only recognized fibrillarin pretreated with HgCl2 (Hg2+ fibrillarin), suggesting that Hg2+ can induce the presentation of a novel fibrillarin epitope. Four fibrillarin-specific hybridoma clones studied for cytokine production were shown to produce interleukin (IL)-2, and three of them also produced IL-4. For stimulation of fibrillarin-specific T cell hybridomas, exogenous murine fibrillarin had to be added when antigen-presenting cells (APCs) came from untreated mice, but not when the APCs were obtained from HgCl2-treated animals. Apparently, HgCl2 treatment induces the presentation by APCs of a novel Hg2+ fibrillarin epitope and up-regulates the presentation of unaltered fibrillarin epitopes, thus activating 'Hg(2+)-specific' as well as autoreactive CD4+ T cells. |
| Databáze: | OpenAIRE |
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