An automated system using spatial oversampling for optical mapping in murine atria. Development and validation with monophasic and transmembrane action potentials

Autor: Benjamin Osborne, Paulus Kirchhof, Ting Y. Yu, Fahima Syeda, Keith L. Brain, Hamid Dehghani, Andrew P. Holmes, Larissa Fabritz
Rok vydání: 2014
Předmět:
Optical mapping
Conduction velocity
Neural Conduction
LA
Left atrium
Action Potentials
CL
Cycle length
030204 cardiovascular system & hematology
Isolated atrium
CV
Conduction velocity
Pattern Recognition
Automated
Mice
0302 clinical medicine
Photography
AF
Atrial fibrillation
Oversampling
Voltage-Sensitive Dye Imaging
Original Research
Physics
0303 health sciences
ADC
Analogue to digital converter
Signal Processing
Computer-Assisted
APD
Action potential duration
Transmembrane protein
cardiovascular system
Electrical conduction system of the heart
MAP
Monophasic action potential
TAP
Transmembrane action potential
Biophysics
LED
Light emitting diode
TIFF
Tagged image file format
Sensitivity and Specificity
03 medical and health sciences
Heart Conduction System
Animals
CMOS
Complementary metal-oxide-semiconductor
Molecular Biology
030304 developmental biology
OAP
Optical action potential
Reproducibility of Results
Atrial action potential duration
Atrial fibrillation
SEM
Standard error of the mean
Murine model
Sample Size
Temporal resolution
Biomedical engineering
Zdroj: Progress in Biophysics and Molecular Biology
ISSN: 0079-6107
DOI: 10.1016/j.pbiomolbio.2014.07.012
Popis: We developed and validated a new optical mapping system for quantification of electrical activation and repolarisation in murine atria. The system makes use of a novel 2nd generation complementary metal-oxide-semiconductor (CMOS) camera with deliberate oversampling to allow both assessment of electrical activation with high spatial and temporal resolution (128 × 2048 pixels) and reliable assessment of atrial murine repolarisation using post-processing of signals. Optical recordings were taken from isolated, superfused and electrically stimulated murine left atria. The system reliably describes activation sequences, identifies areas of functional block, and allows quantification of conduction velocities and vectors. Furthermore, the system records murine atrial action potentials with comparable duration to both monophasic and transmembrane action potentials in murine atria.
Databáze: OpenAIRE
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