Prostaglandins and cyclic nucleotides in moloney sarcoma tumors
| Autor: | John L. Humes, Helen R. Strausser |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Prostaglandin Antagonists medicine.medical_treatment Indomethacin Radioimmunoassay Prostaglandin Arachidonic Acids In Vitro Techniques Biochemistry Mice chemistry.chemical_compound Endocrinology Internal medicine Cyclic AMP medicine Animals Nucleotide Cyclic GMP Incubation chemistry.chemical_classification Muscles medicine.disease Stimulation Chemical Thin-layer chromatography In vitro Hindlimb Bucladesine chemistry Chromatography Gel Prostaglandins lipids (amino acids peptides and proteins) Arachidonic acid Chromatography Thin Layer Sarcoma Experimental Sarcoma Moloney murine leukemia virus Prostaglandin E |
| Zdroj: | Prostaglandins. 5:183-196 |
| ISSN: | 0090-6980 |
| Popis: | A 53-fold increase in the amount of prostaglandin E as well as a 7-fold increase in the amount of prostaglandin F was found associated with virus-induced Moloney sarcoma tumors when compared with the amounts found in normal leg muscles from weanling BALB/c mice. There was a concomitant 2-fold increase in the concentration of cyclic AMP, however, no significant change in the concentration of cyclic GMP was observed. Prostaglandin E 1 stimulated cyclic AMP formation in tumor tissue incubated in vitro . The tumor tissue continued to synthesize relatively large amounts of prostaglandin E during a 3 hr. incubation. The prostaglandins were primarily recovered in the incubation medium and not retained in the tissue. Indomethacin, an established prostaglandin synthetase inhibitor, prevented the synthesis. Arachidonic acid and dibutyryl-cyclic AMP failed to stimulate this synthesis. The prostaglandin of the E series associated with the tumor was identified as prostaglandin E 2 by thin layer chromatography. |
| Databáze: | OpenAIRE |
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