Germline RNA helicases couple RNA binding to P granule assembly at nuclear periphery
| Autor: | Yabing Hu, Charles F. Lang, Wenjun Chen, Ed Munro, Jordan S. Brown, Xiaoyan Song, Heng-Chi Lee, Karen Bennett, Donglei Zhang |
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| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Mutation Chemistry Mutant RNA P granule assembly RNA-binding protein medicine.disease_cause RNA Helicases Germline 3. Good health Cell biology 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure medicine 030217 neurology & neurosurgery Germ cell 030304 developmental biology |
| DOI: | 10.1101/760256 |
| Popis: | P granules are phase-separated liquid droplets that play important roles in the maintenance of the germ cell fate in C. elegans. The localization and formation of P granules are highly dynamic, but mechanisms that regulate such processes remain poorly understood. Here we show that germline RNA helicases (GLHs) control the formation and disassembly of germ granules through their binding and release of RNAs, respectively. In addition, the FGG repeats in the GLHs promote the formation of germ granules at the perinucleus. Proteomic analyses of a mutation that traps RNA-bound GLH-1 complex revealed transient interactions of GLH-1 with several Argonautes and RNA binding proteins. Finally, we found that defects in perinuclear P granule formation correlate with the fertility defects observed in various GLH mutants. Together, our results highlight the versatile roles of RNA helicases in controlling the formation of liquid droplets in space and time. |
| Databáze: | OpenAIRE |
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