Identification of novel vaccine candidates for Chagas’ disease by immunization with sequential fractions of a trypomastigote cDNA expression library
| Autor: | Valeria Tekiel, Miriam Postan, Catalina D. Alba-Soto, Daniel O. Sánchez, Stella M. González Cappa |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Protozoan Vaccines
Chagas disease DNA Complementary Trypanosma cruzi CIENCIAS MÉDICAS Y DE LA SALUD Trypanosoma cruzi Inmunología Antibodies Protozoan Expression Library Immunization Ciencias Biológicas Mice Antigen Biología Celular Microbiología Complementary DNA parasitic diseases medicine Animals Humans Chagas Disease Gene Gene Library Mice Inbred C3H Tripomastigote antigens General Veterinary General Immunology and Microbiology biology Public Health Environmental and Occupational Health Kinetoplastida DNA Protozoan Bioquímica y Biología Molecular biology.organism_classification medicine.disease Virology Medicina Básica Latin America Infectious Diseases Immunization Antibody Formation Molecular Medicine Trypanosomiasis Vaccine CIENCIAS NATURALES Y EXACTAS |
| Popis: | The protozoan Trypanosoma cruzi is the etiological agent of Chagas´ disease, a major chronic infection in Latin America. Currently, there are neither effective drugs nor vaccines for the treatment or prevention of the disease. Several T. cruzi surface antigens are being tested as vaccines but none of them proved to be completely protective, probably because they represent only a limited repertoire of all the possible T. cruzi target molecules. Taking into account that the trypomastigote stage of the parasite must express genes that allow the parasite to disseminate into the tissues and invade cells, we reasoned that genes preferentially expressed in trypomastigotes represent potential targets for immunization. Here we screened an epimastigote-subtracted trypomastigote cDNA expression library by genetic immunization, in order to find new vaccine candidates for Chagas´ disease. After two rounds of immunization and challenge with trypomastigotes, this approach led to the identification of a pool of 28 gene fragments that improved in vivo protection. Sequence analysis of these putative candidates revealed that 19 out of 28 (67.85%) of the genes were hypothetical proteins or unannotated T. cruzi open reading frames, which certainly would not have been identified by other methods of vaccine discovery. Fil: Alba Soto, Catalina Dirney. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Tekiel, Valeria Sonia. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: González, Stella Maris. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Postan, Miriam. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina Fil: Sanchez, Daniel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina |
| Databáze: | OpenAIRE |
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