Cuticular fatty acid profile analysis of three Rhipicephalus tick species (Acari: Ixodidae)
| Autor: | Oran Erster, Jakob Avi Shimshoni, Stefan Soback, Olga Cuneah, Asael Rot, V. Shkap |
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| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Zoology Tick 01 natural sciences Gas Chromatography-Mass Spectrometry 03 medical and health sciences Abundance (ecology) Botany Rhipicephalus Animals Acari Relative species abundance 030304 developmental biology chemistry.chemical_classification 0303 health sciences Ecology biology Fatty Acids Fatty acid General Medicine biology.organism_classification 010602 entomology chemistry Animal ecology Insect Science Female lipids (amino acids peptides and proteins) Ixodidae |
| Zdroj: | Experimental and Applied Acarology. 61:481-489 |
| ISSN: | 1572-9702 0168-8162 |
| Popis: | Cuticular fatty acids (CFA) are important constituents of the arthropod exoskeleton, serving as structural and defense components, and participating in intra-species communication. Here we describe for the first time a comparative analysis of the CFA profiles of three tick species of the genus Rhipicephalus: R. annulatus, R. bursa and R. sanguineus. CFA profiles were determined for R. bursa and R. sanguineus grown both on rabbit or calf, and for R. annulatus grown on calf. CFA composition was compared for each species before and after ethanol treatment, for different hosts of each species, and between the different species. Our data suggest that adsorption of the host's fatty acids changes the apparent CFA composition. Ethanol treatment efficiently removed the unbound fatty acids from the ticks and revealed the actual composition. Comparison between ticks grown on rabbit versus calf showed significant difference in the relative abundance of fatty acids C14 and 9,12-C18:2 for R. bursa, and a difference in the relative abundance of C14 for R. sanguineus. Comparison of the CFA between the three species revealed significant differences in the abundance of fatty acids C16, 9,12-C18:2, 9-C18:1, C18 and C20. Our results show that while the host had a minor effect on CFA composition within each species, significant differences were observed in the CFA profiles of different species. We suggest that CFA profiles may be used to distinguish between related species. CFA analysis can also be used in studies of communication and defense mechanisms in ticks and other arthropods. |
| Databáze: | OpenAIRE |
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