Antiepileptic effect of acylpolyaminetoxin JSTX-3 on rat hippocampal CA1 neurons in vitro
| Autor: | Katsuhiro Konno, Gianina Teribele Venturin, Ken-ichi Nihei, Fernanda de Borba Cunha, Mario Sergio Palma, Raquel Mattos de Oliveira, Simone Denise Salamoni, Daniela Souza de Abreu, Jaderson Costa da Costa, Andrea Luisa Alencar Tavares, Ricardo Vaz Breda |
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| Rok vydání: | 2005 |
| Předmět: |
Male
N-Methylaspartate Patch-Clamp Techniques Action Potentials Hippocampus In Vitro Techniques Biology Pharmacology Hippocampal formation Heterocyclic Compounds Excitatory Amino Acid Agonists Polyamines medicine Animals Magnesium Rats Wistar Molecular Biology Neurons Epilepsy General Neuroscience Glutamate receptor N-Methylscopolamine Spider toxin Electric Stimulation Rats medicine.anatomical_structure Animals Newborn nervous system Pilocarpine Excitatory postsynaptic potential NMDA receptor Anticonvulsants Neurology (clinical) Neuron Neuroscience Developmental Biology medicine.drug |
| Zdroj: | Brain Research. 1048:170-176 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2005.04.060 |
| Popis: | The Joro spider toxin (JSTX-3), derived from Nephila clavata, has been found to block glutamate excitatory activity. Epilepsy has been studied in vitro, mostly on rat hippocampus, through brain slices techniques. The aim of this study is to verify the effect of the JSTX-3 on the epileptiform activity induced by magnesium-free medium in rat CA1 hippocampal neurons. Experiments were performed on hippocampus slices of control and pilocarpine-treated Wistar rats, prepared and maintained in vitro. Epileptiform activity was induced through omission of magnesium from the artificial cerebrospinal fluid (0-Mg2+ ACSF) superfusate and iontophoretic application of N-methyl-D-aspartate (NMDA). Intracellular recordings were obtained from CA1 pyramidal neurons both of control and epileptic rats. Passive membrane properties were analyzed before and after perfusion with the 0-Mg2+ ACSF and the application of toxin JSTX-3. During the ictal-like activity, the toxin JSTX-3 was applied by pressure ejection, abolishing this activity. This effect was completely reversed during the washout period when the slices were formerly perfused with artificial cerebrospinal fluid (ACSF) and again with 0-Mg2+ ACSF. Our results suggest that the toxin JSTX-3 is a potent blocker of induced epileptiform activity. |
| Databáze: | OpenAIRE |
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