Extracellular insulin degrading activity creates instability in a CHO-based batch-refeed continuous process
| Autor: | S. R. Adamson, D. C. Cohen, Martin S. Sinacore, Yen-Tung Luan, Denis Drapeau, D. T. Richards, J. A. Popoloski |
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| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Time Factors Cell division Cell Survival medicine.medical_treatment Clinical Biochemistry Biomedical Engineering Hamster Bioengineering CHO Cells Biology Insulysin Internal medicine Cricetinae Culture Techniques medicine Extracellular Animals Humans Insulin Viability assay Cell growth Chinese hamster ovary cell Macrophage Colony-Stimulating Factor Cell Biology Recombinant Proteins Tetrahydrofolate Dehydrogenase Endocrinology Cell culture Cell Division Biotechnology |
| Zdroj: | Cytotechnology. 15(1-3) |
| ISSN: | 0920-9069 |
| Popis: | In a batch-refeed continuous process involving a recombinant Chinese hamster ovary cell line, a brief upset was occasionally observed during which cell growth halted and cell viability dropped. This was found to be associated with depletion of insulin from the medium early during the affected passage. Insulin depletion was found to be primarily the result of insulin degrading activity released by the cells during the preceding passage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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