Lower HIV Provirus Levels Are Associated with More APOBEC3G Protein in Blood Resting Memory CD4+ T Lymphocytes of Controllers In Vivo
| Autor: | Maria Pia De Pasquale, Yordanka Kourteva, Tara M. Allos, Richard T. D'Aquila |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
CD4-Positive T-Lymphocytes
viruses Gene Expression lcsh:Medicine HIV Infections Viremia APOBEC-3G Deaminase Adaptive Immunity Biology Peripheral blood mononuclear cell 03 medical and health sciences Proviruses T-Lymphocyte Subsets Cytidine Deaminase vif Gene Products Human Immunodeficiency Virus medicine Humans lcsh:Science APOBEC3G HLA-B27 Antigen Immunodeficiency 030304 developmental biology Infectivity 0303 health sciences Multidisciplinary 030306 microbiology lcsh:R Virion Viral Load biochemical phenomena metabolism and nutrition Provirus medicine.disease Acquired immune system Virology 3. Good health HLA-B Antigens Host-Pathogen Interactions Immunology HIV-1 lcsh:Q Immunologic Memory Viral load Research Article |
| Zdroj: | PLoS ONE, Vol 8, Iss 10, p e76002 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0076002 |
| Popis: | Immunodeficiency does not progress for prolonged periods in some HLA B57- and/or B27-positive subjects with human immunodeficiency virus type 1 (HIV) infection, even in the absence of antiretroviral therapy (ART). These “controllers” have fewer HIV provirus-containing peripheral blood mononuclear cells than “non-controller” subjects, but lymphocytes that harbor latent proviruses were not specifically examined in studies to date. Provirus levels in resting memory cells that can serve as latent reservoirs of HIV in blood were compared here between controllers and ART-suppressed non-controllers. APOBEC3G (A3G), a cellular factor that blocks provirus formation at multiple steps if not antagonized by HIV virion infectivity factor (Vif), was also studied. HLA-linked HIV control was associated with less provirus and more A3G protein in resting CD4+ T central memory (Tcm) and effector memory (Tem) lymphocytes (provirus: p = 0.01 for Tcm and p = 0.02 for Tem; A3G: p = 0.02 for Tcm and p = 0.02 for Tem). Resting memory T cells with the highest A3G protein levels (>0.5 RLU per unit of actin) had the lowest levels of provirus ( |
| Databáze: | OpenAIRE |
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