Detection ofMycobacterium avium-intracellulareComplex in Bone Marrow Specimens of Patients with Acquired Immunodeficiency Syndrome
| Autor: | Stephen D. Nightingale, Arie Perry, Elizabeth L. Wiley, Jean Lawrence |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Opportunistic infection Colony Count Microbial Bone Marrow Biopsy medicine Humans Histiocyte Acquired Immunodeficiency Syndrome Clinical Trials as Topic Mycobacterium bovis Staining and Labeling biology medicine.diagnostic_test business.industry General Medicine Mycobacterium avium Complex medicine.disease biology.organism_classification medicine.anatomical_structure Granuloma Bacteremia Female Gentamicin Bone marrow Gentamicins business medicine.drug |
| Zdroj: | American Journal of Clinical Pathology. 101:446-451 |
| ISSN: | 1943-7722 0002-9173 |
| DOI: | 10.1093/ajcp/101.4.446 |
| Popis: | Thirty-seven bone marrow core biopsy specimens from 21 human immunodeficiency virus-infected patients with Mycobacterium avium-intracellulare complex bacteremia were stained using rabbit polyclonal antibodies against Mycobacterium bovis strain Bacillus-Calmette-Guerin (BCG) and Mycobacterium duvalii , as well as Kenyon and Fite stains, to compare sensitivities of these techniques and evaluate possible response to therapy. The patients in this study had participated in a phase I/II trial of liposome-encapsulated gentamicin therapy. Two biopsy specimens had inadequate tissue for evaluation. Thirtytwo specimens demonstrated bacilli with anti- M duvalii , 33 with anti- BCG, 20 with Kenyon, and 23 with Fite. Two were negative with all stains. Fifteen biopsy specimens had epithelioid granulomas, 12 had histiocytic granulomas, and 1 had a granuloma of indeterminate type. The remaining seven biopsy specimens had no granulomas. Four of these seven demonstrated bacilli with anti- M duvalii , 5 with anti-BCG, 1 with Kenyon, and 2 with Fite. The number of M avium-intracellulare organisms per milliliter of blood decreased in 14 of 21 patients after liposome-encapsulated gentamicin therapy. However, none of the 11 patients whose pre- and posttherapy bone marrow core biopsy specimens were both evaluable demonstrated a reduction in the number of M avium-intracellulare organisms. The authors concluded that anti- M duvalii and anti-BCG are more sensitive than acid-fast stains for identifying M avium-intracellulare infection in bone marrow core biopsy specimens of patients who have acquired immunodeficiency syndrome (AIDS) with M avium-intracellulare bacteremia. Bone marrow core biopsy specimens may provide a perspective on M avium-intracellulare infection in AIDS patients that differs from the one provided by blood cultures. |
| Databáze: | OpenAIRE |
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