Syntheses of 18F-Labeled Acyclic Purine and Pyrimidine Nucleosides Intended for Monitoring Gene Expression

Autor:	St. Noll, Michaela Grote, Bernhard Noll
Jazyk:	angličtina
Rok vydání:	2004
Předmět:	Purine medicine.diagnostic_test Pyrimidine High-performance liquid chromatography HSV-1 tk chemistry.chemical_compound PET chemistry Biochemistry Positron emission tomography Gene expression medicine fluorine-18 gene expression Specific activity Physical and Theoretical Chemistry Gene Nucleoside
Zdroj:	European Symposium on Radiopharmacy and Radiopharmaceuticals, 09.-12.09.2004, Danzig, Poland Radiochimica Acta 93(2005)9-10, 585-588 6th International Conference on Nuclear and Radiochemistry, 29.08.-03.09.2004, Aachen, Germany
Popis:	Summary Non-invasive imaging of genes which are introduced into cells is a useful method for gene therapy monitoring. The labeling of acyclic purine and pyrimidine nucleoside derivatives with fluorine-18 required for the HSV-1 tk imaging approach with positron emission tomography (PET) is described. The methoxytritylated and tosylated precursors were radiolabeled using a K[18F]F/kryptofix 2.2.2™ complex, followed by removal of the protecting groups under acidic conditions and HPLC purification. The radiochemical yields of the 18F-tracers amount to 5–15% (decay corrected) after a synthesis time of 85–95 min; the radiochemical purity was >98% with an average specific activity of 19 GBq/μmol at the end of synthesis.
Databáze:	OpenAIRE
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