Production and characterization of chitooligosaccharides by the fungal chitinase Chit42 immobilized on magnetic nanoparticles and chitosan beads: selectivity, specificity and improved operational utility
| Autor: | Kidibule, Peter E., Costa, Jessica, Atrei, Andrea, Plou, Francisco J., Pogni, Rebecca, Fernández Lobato, María |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Ministerio de Ciencia e Innovación (España), Fundación Ramón Areces, UAM. Departamento de Biología Molecular |
| Rok vydání: | 2021 |
| Předmět: |
magnetic nanoparticles
chitosan beads Colloidal Chitin Immobilized enzyme General Chemical Engineering Fungal Chitinase ensymatic immobilization macromolecular substances Chitooligosaccharides Chitosan chemistry.chemical_compound Hydrolysis Hydrolytic Activities Operational Stability Operational Utility Increasing Temperatures biology Reaction Mixture Fungal Chitinase ensymatic immobilization magnetic nanoparticles chitosan beads Chitooligosaccharides General Chemistry Biología y Biomedicina / Biología equipment and supplies Combinatorial chemistry carbohydrates (lipids) chemistry Immobilized Enzyme Chitinase Genipin biology.protein Magnetic nanoparticles Glutaraldehyde Selectivity |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Biblos-e Archivo. Repositorio Institucional de la UAM |
| ISSN: | 2016-7660 |
| Popis: | Chitin-active enzymes are of great biotechnological interest due to the wide industrial application of chitinolytic materials. Non-stability and high cost are among limitations that hinder industrial application of soluble enzymes. Here we report the production and characterization of chitooligosaccharides (COS) using the fungalexo-chitinase Chit42 immobilized on magnetic nanoparticles and food-grade chitosan beads with an immobilization yield of about 60% using glutaraldehyde and genipin linkers. The immobilized enzyme gained operational stability with increasing temperature and acidic pH values, especially when using chitosan beads-genipin that retained more than 80% activity at pH 3. Biocatalysts generated COS from colloidal chitin and different chitosan types. The immobilized enzyme showed higher hydrolytic activity than free enzyme on chitosan, and produced COS mixtures with higher variability of size and acetylation degree. In addition, biocatalysts were reusable, easy to handle and to separate from the reaction mixture. Spanish Ministry of Economy and Competitiveness [BIO2016-76601-C3-1/-2], the Spanish Ministry of Science and Innovation PID2019-105838RB-C32/-31, Fundación Ramón Areces |
| Databáze: | OpenAIRE |
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