Affinity electrophoresis of human serum alkaline phosphatase isoenzymes in agarose gel containing lectin
| Autor: | Doina Onica, Lars Sundblad, Lennart Waldenlind |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Clinical Biochemistry
Phosphatase Biochemistry Isozyme Bone and Bones chemistry.chemical_compound Lectins Humans Electrophoresis Agar Gel Gel electrophoresis biology Biochemistry (medical) Lectin General Medicine Alkaline Phosphatase Molecular biology Intestines Isoenzymes Electrophoresis Liver chemistry biology.protein Agarose Alkaline phosphatase Affinity electrophoresis Densitometry |
| Zdroj: | Clinica Chimica Acta. 155:285-293 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/0009-8981(86)90248-2 |
| Popis: | Separation of alkaline phosphatase isoenzymes using affinity electrophoresis in agarose gel containing lectin is described. The bone and biliary isoenzymes precipitate during electrophoresis and are clearly separated from the liver isoenzyme. The liver, intestinal and placental alkaline phosphatases are essentially not affected by the lectin. The migration distances of the precipitating bone and biliary fractions vary with their alkaline phosphatase activity. The bone isoenzyme is more heterogeneous than the biliary isoenzyme with respect to interaction with lectin forming both insoluble and soluble complexes. Affinity electrophoresis in agarose gel containing lectin can be used for quantitation by densitometry of liver and bone isoenzymes in sera containing only these two fractions but must be combined with conventional electrophoresis, preferably in agar gel, if biliary, intestinal, or placental isoenzymes are also present. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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