Combination of yeast hydrolysates to improve CHO cell growth and IgG production
| Autor: | Mathilde Mosser, Eric Olmos, Fabrice Blanchard, Romain Kapel, Ivan Marc, Annie Marc, Eric Oriol, Isabelle Chevalot |
|---|---|
| Přispěvatelé: | Immuno-Endocrinologie Cellulaire et Moléculaire [Nantes] (IECM), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Laboratoire Réactions et Génie des Procédés (LRGP), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Sciences du Génie Chimique (LSGC), Institut National Polytechnique de Lorraine (INPL)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL) |
| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
Clinical Biochemistry Biomedical Engineering Bioreactor Bioengineering Biology 01 natural sciences Hydrolysate law.invention 03 medical and health sciences [SPI]Engineering Sciences [physics] Erlenmeyer flask law 010608 biotechnology Yeast extract [CHIM]Chemical Sciences [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering CHO cell culture ComputingMilieux_MISCELLANEOUS 030304 developmental biology Original Research 0303 health sciences Cell growth Chinese hamster ovary cell IgG recombinant protein Cell Biology Yeast Chemically defined medium Biochemistry Cell culture Yeast peptone Kinetic studies Biotechnology |
| Zdroj: | Cytotechnology Cytotechnology, Springer Verlag, 2013, 65 (4), pp.629-641. ⟨10.1007/s10616-012-9519-1⟩ Cytotechnology, Springer Verlag, 2012, 65, pp.629-641. ⟨10.1007/s10616-012-9519-1⟩ |
| ISSN: | 0920-9069 1573-0778 |
| Popis: | International audience; Many studies underlined the great benefits of hydrolysates used as additives in animal free media on cell culture performances. However, to precisely define hydrolysate supplementation strategies, a deeper understanding of their effect on cell growth and protein production is required. In the present study, the effect of addition of one yeast extract (YE) and two yeast peptones (named YP.A and YP.B) in a chemically defined medium was first assessed on cell culture performances. Interestingly, specific effects were found depending on the degree of degradation of yeast hydrolysates. The YE at 1 g L-1 increased the maximal cell density by 70 %, while a mixture of YE (1 g L-1) and YP.A (4 g L-1) increased IgG production by 180 %. These conditions were then evaluated on the CHO cell kinetics all over cultures. Hydrolysates extended the cell growth phase in Erlenmeyer flask and increased the maximal growth rate in bioreactor up to 20 %. Cell growth stimulation induced by hydrolysates addition was linked with energetic metabolism improvement suggesting that they promote oxidative pathway. Furthermore, hydrolysates provided an additional source of substrate that supported cell growth despite glutamine limitation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink