Role of counter-ion and helper lipid content in the design and properties of nanocarrier systems : a biophysical study in 2D and 3D lipid assemblies
| Autor: | Carlos Rodríguez-Abreu, Pedro Alpuim, Marcos Cerqueira, M. E. C. D. Real Oliveira, Andreia C. Gomes, Gerald Brezesinski, Sara S. Nogueira, Ana C. Oliveira, J. Tovar, Odete Gonçalves, Marlene Lúcio |
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| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
chemistry.chemical_classification
Langmuir Science & Technology Brewster's angle Ciências Naturais::Ciências Físicas Chemistry Small-angle X-ray scattering General Chemical Engineering Ciências Físicas [Ciências Naturais] Cationic polymerization 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Homogeneous distribution 0104 chemical sciences symbols.namesake Crystallography Membrane Chemical engineering symbols Counterion 0210 nano-technology Raman spectroscopy |
| Zdroj: | RSC Advances Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | There is a direct correlation between the physicochemical properties of nanocarrier systems and their biological performance, including stability under physiological conditions, cellular internalization and transfection efficiency. Therefore, understanding the biophysical aspects that affect self-assembled nanocarriers is determinant for a rational design of efficient formulations. In this study, a comprehensive evaluation of the effects of each component on the molecular organization of aggregates formed by the cationic lipids dioctadecyldimethylammonium bromide and chloride (DODAB and DODAC) and the neutral lipid monoolein (MO) was made. Specifically, the effects of the helper lipid content (MO) and the role of the counter-ion of the cationic lipids were evaluated in 2D and 3D assemblies by Langmuir surface pressure–molecular area (π–A) isotherms, Brewster Angle Microscopy (BAM), infrared reflection absorption spectroscopy (IRRAS), confocal Raman microscopy, and Small Angle X-ray Scattering (SAXS). The results show that MO has a different distribution on the DODAC and DODAB bilayers, and a fluidizing effect dependent on the MO content. For low MO molar ratios, the fluidizing effect was more pronounced in DODAC : MO mixtures, indicating a more homogeneous distribution of MO in DODAC than in DODAB bilayers. For high MO molar ratios, packing of membranes was similar for both cationic lipids, and the effect of the counter-ion is attenuated. The distribution of MO in the two cationic systems is closely related with the efficiency of the counter-ions in the screening of the charged group. We acknowledge DAAD/FCT that provided the financial support required to gather the Portuguese and the German coworkers. This work was further supported by FEDER through POFC-COMPETE and by national funds from FCT, through the projects PEst-OE/BIA/UI4050/2014 (CBMA) and PEst-C/FIS/UI0607/2013 (CFUM). Marlene Lúcio acknowledges FCT for the financial support provided by the exploratory project IF/00498/2012. C.R.-A. is grateful to the European Union through the Operational Programme for Cross-border Cooperation: Spain-Portugal under Grant POCTEP 2007-2013 and to European Regional Development Fund for research funding (Innovation in Nanomedicine Project). The authors would also like to acknowledge Irina Berndt and Claudia Botelho. |
| Databáze: | OpenAIRE |
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