Increased susceptibility to urethane-induced lung tumors in mice with decreased expression of connexin43
Autor: | Sílvia Catarina Salgado Oloris, José Luis Avanzo, Maria Lúcia Zaidan Dagli, Ivone Isabel Mackowiak, Marc Mesnil, Silvia Maria Gomes Massironi, Claudia Madalena Cabrera Mori, Francisco Javier Hernandez-Blazquez, Hiroshi Yamasaki, Ana Paula Gárate, Tereza Cristina da Silva |
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Rok vydání: | 2004 |
Předmět: |
Adenoma
Cancer Research medicine.medical_specialty Pathology Heterozygote Lung Neoplasms Ratón Heterologous Connexin Biology medicine.disease_cause Urethane S Phase Mice Internal medicine medicine Animals Carcinogen Mice Knockout Lung Gap junction General Medicine medicine.disease Mice Inbred C57BL Endocrinology medicine.anatomical_structure Connexin 43 cardiovascular system Carcinogens sense organs Disease Susceptibility biological phenomena cell phenomena and immunity Carcinogenesis Gene Deletion |
Zdroj: | Carcinogenesis. 25(10) |
ISSN: | 0143-3334 |
Popis: | Gap junction intercellular communication capacity and connexin expression are reportedly decreased in human lung cancer. The mechanisms by which connexins, the gap junction proteins, act as tumor suppressors are unclear. In order to understand the involvement of connexins in tumorigenesis, we analyzed the effect of the heterologous deletion of Gja1 [the connexin43 (Cx43) gene] on the development of lung adenomas in mice. Heterozygous (Cx43(+/-)) and wild-type mice (Cx43(+/+)) were treated or not with single doses of urethane at 15 and 17 days after birth. Twenty-five weeks later, both the number and size of nodules were increased in Cx43(+/-) mice as compared with Cx43(+/+) mice. Moreover, the lesions were histologically more aggressive in the heterozygous mice. However, no increase in spontaneous lesions was observed in the lungs of untreated Cx43(+/-) mice. Heterozygous mice effectively presented lower expression of Cx43 genes and decreased amounts of Cx43. In conclusion, our results indicate that deletion of one allele of the Cx43 gene clearly favors the carcinogenic effect of urethane administration and results in a higher susceptibility to lung adenoma formation in mice. |
Databáze: | OpenAIRE |
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