Rapid and sensitive detection of ketamine in blood using novel fluorescence genosensor
| Autor: | Jiang Ling, Lingmei Lan, Jie Yan, Xingmei Li, Jifeng Cai, Weichen Li, Lagabaiyla Zha, Yunfeng Chang, Yanjun Ding, Yadong Guo |
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| Rok vydání: | 2017 |
| Předmět: |
Silver
Analytical chemistry Metal Nanoparticles 02 engineering and technology Biosensing Techniques 010402 general chemistry 01 natural sciences Biochemistry Rapid detection Analytical Chemistry Drug detection Drug Users medicine Humans Ketamine Forensic examination Detection limit Analgesics Chromatography Chemistry DNA 021001 nanoscience & nanotechnology Fluorescence Quantitative determination Public attention 0104 chemical sciences 0210 nano-technology medicine.drug |
| Zdroj: | Analytical and bioanalytical chemistry. 409(30) |
| ISSN: | 1618-2650 |
| Popis: | In recent years, drug abuse has been considered as a most challenging social problem that aroused public attention. Ketamine has increased in unregulated use as a ‘recreational drug’ in teenagers. However, there is no suitable and maneuverable detection method for ketamine in situ at the moment. Fluorescence sensor technique, with predominant recognition and simple operation, is a good potential application in drug detection. Here, we first reported a highly sensitive and selective fluorescence genosensor for rapid detection of ketamine based on DNA-templated silver nanoclusters (DNA-AgNCs) probes, in which the DNA sequence could specially recognize ketamine with high affinity. Parameters affecting detection efficiency were investigated and optimized. Under optimum conditions, the as-prepared genosensor can allow for the determination of ketamine in the concentration range of 0.0001–20 μg/mL with two linear equations: one is y = 2.84x-7.139 (R2 = 0.987) for 0.0001–0.1 μg/mL, and the other is y = 1.87x-0.091 (R2 = 0.962) for 0.1–20 μg/mL, and the estimated detection limit of ketamine is 0.06 ng/mL. Moreover, the feasibility of this proposed method was also demonstrated by analyzing forensic blood samples. Compared with official gas chromatography/mass spectrometry (GC/MS), this fluorescence genosensor is simple, rapid, and accurate for quantitative determination of ketamine in blood for pharmaceutical and forensic analysis. Overall, it is the first report on a fluorescence genosensor for detecting ketamine directly in blood. This research may provide a new insight for the analyst to band fluorescence genosensor technology together with drug monitoring in the battle against drug abuse and forensic examination. |
| Databáze: | OpenAIRE |
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