A poliomyelitis model through mucosal infection in transgenic mice bearing human poliovirus receptor, TgPVR21
| Autor: | Satoshi Koike, Ayako Harashima, Yasushi Ami, Tetsutaro Sata, Akio Nomoto, Takuya Iwasaki, Takao Yoshii, Noriyo Nagata, Tsutomu Hashikawa, Yuriko Suzaki, Ikuyoshi Hatano, Yuko Sato, Takeshi Kurata, Yoshinobu Horiuchi |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Central Nervous System
Male Time Factors Flaccid paralysis viruses Mice Transgenic Antibodies Viral medicine.disease_cause complex mixtures Microbiology Mice Virology medicine Paralysis Animals Animal model Antigens Viral Administration Intranasal TgPVR21 biology Infectious dose Poliovirus Intranasal inoculation Mucosal infection Brain Membrane Proteins Immunohistochemistry Mucosal Infection Disease Models Animal Nasal Mucosa Spinal Cord Viral replication Poliovirus Vaccine Oral biology.protein Receptors Virus Female Nasal administration Disease Susceptibility Antibody medicine.symptom Poliomyelitis |
| Zdroj: | Virology. 321:87-100 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2003.12.008 |
| Popis: | Transgenic mice bearing the human poliovirus receptor (TgPVR) are less susceptible to oral inoculation, although they are susceptible to parenteral inoculation. We investigated the susceptibility of TgPVR 21 line [Arch. Virol. 130 (1994) 351] to poliovirus through various mucosal routes. Intranasal inoculation of a neurovirulent Mahoney strain (OM1) caused flaccid paralysis with viral replication in the central nervous system at a dose of 106 cell culture infectious dose (CCID50), in contrast, no paralysis following oral or intragastric inoculation of the same dose. Intranasal inoculation of a vaccine strain, Sabin 1, at 106 CCID50, resulted in no paralysis. Initial replication of poliovirus in the nasal cavity was confirmed by virus isolation and detection of negative-stranded replicative intermediates by RT-PCR and viral antigens using a high-sensitive immunohistochemistry and genome/transcripts by in situ hybridization. Poliovirus-specific IgG antibodies were elevated in the sera of surviving TgPVR21. This model can be used as a mucosal infection model and for differentiation of neurovirulent and attenuated poliovirus strains. |
| Databáze: | OpenAIRE |
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