Isolation and Study of Temperature-sensitive Mutants of Rabies Virus
| Autor: | Françoise Bussereau, Jacqueline Benejean, Naima Saghi |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Genes
Viral Mutant Nitrous Acid Mutagen Viral Plaque Assay Biology medicine.disease_cause Virus chemistry.chemical_compound Virology medicine Genetic Complementation Test Rabies virus Temperature medicine.disease Molecular biology Phenotype Complementation chemistry Ethyl Methanesulfonate Mutation Agarose Rabies Fluorouracil |
| Zdroj: | Journal of General Virology. 60:153-158 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-60-1-153 |
| Popis: | Summary One-hundred and seventeen temperature-sensitive (ts) mutants have been isolated from the challenge virus strain (CVS) of rabies virus (RV). The criterion used for this selection was the absence of plaque-forming units on CER cells under agarose medium at the non-permissive temperature (NPT) of 38.5 °C. Of these mutants, 102 were induced by 5-fluorouracil. This compound was much more effective as a mutagen than either nitrous acid or the alkylating agents ethyl methanesulphonate and methyl methanesulphonate. Seventy-six of the ts mutants exhibited residual multiplication at NPT which was less than 2% of that of the wild-type virus. Attempts to demonstrate complementation were unsuccessful, although three phenotypic groups have been established by biochemical and immunological tests. Possible reasons for the failure to obtain complementation are discussed. |
| Databáze: | OpenAIRE |
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