The Integral Membrane Protein SEN1 is Required for Symbiotic Nitrogen Fixation in Lotus japonicus Nodules
| Autor: | Satoshi Tabata, Michael K. Udvardi, Yasukazu Nakamura, Takeshi Yamamoto, Thomas Rørby Petersen, Hirotaka Kumagai, Jens Stougaard, Tsuneo Hakoyama, Hiroshi Kouchi, Katja Brossuleit, Shusei Sato, Masanori Tamaoki, Kaori Niimi, Sawa Isobe, Niels Sandal, Norio Suganuma, Yosuke Umehara, Masayoshi Kawaguchi |
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| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
Root nodule Physiology Lotus japonicus Plant Science Biology Genes Plant 01 natural sciences Rhizobia 03 medical and health sciences Gene Expression Regulation Plant Nitrogen Fixation Botany medicine Cloning Molecular Symbiosis Phylogeny Plant Proteins 030304 developmental biology 0303 health sciences Genetic Complementation Test Membrane Proteins food and beverages Nitrogenase Nif gene Nodule (medicine) Cell Biology General Medicine biology.organism_classification Phenotype Biochemistry Symbiosome Mutation Lotus Nitrogen fixation medicine.symptom Root Nodules Plant Rhizobium 010606 plant biology & botany |
| Zdroj: | Plant and Cell Physiology Hakoyama, T, Niimi, K, Yamamoto, T, Isobe, S, Sato, S, Nakamura, Y, Tabata, S, Kumagai, H, Umehara, Y, Brossuleit, K, Petersen, T R, Sandal, N, Stougaard, J, Udvardi, M K, Tamaoki, M, Kawaguchi, M, Kouchi, H & Suganuma, N 2012, ' The Integral Membrane Protein SEN1 is Required for Symbiotic Nitrogen Fixation in Lotus japonicus Nodules ', Plant and Cell Physiology, vol. 53, no. 1, pp. 225-36 . https://doi.org/10.1093/pcp/pcr167 |
| ISSN: | 1471-9053 0032-0781 |
| DOI: | 10.1093/pcp/pcr167 |
| Popis: | Legume plants establish a symbiotic association with bacteria called rhizobia, resulting in the formation of nitrogen-fixing root nodules. A Lotus japonicus symbiotic mutant, sen1, forms nodules that are infected by rhizobia but that do not fix nitrogen. Here, we report molecular identification of the causal gene, SEN1, by map-based cloning. The SEN1 gene encodes an integral membrane protein homologous to Glycine max nodulin-21, and also to CCC1, a vacuolar iron/manganese transporter of Saccharomyces cerevisiae, and VIT1, a vacuolar iron transporter of Arabidopsis thaliana. Expression of the SEN1 gene was detected exclusively in nodule-infected cells and increased during nodule development. Nif gene expression as well as the presence of nitrogenase proteins was detected in rhizobia from sen1 nodules, although the levels of expression were low compared with those from wild-type nodules. Microscopic observations revealed that symbiosome and/or bacteroid differentiation are impaired in the sen1 nodules even at a very early stage of nodule development. Phylogenetic analysis indicated that SEN1 belongs to a protein clade specific to legumes. These results indicate that SEN1 is essential for nitrogen fixation activity and symbiosome/bacteroid differentiation in legume nodules. |
| Databáze: | OpenAIRE |
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