A rapid, simple, and sensitive loop-mediated isothermal amplification method to detect toxigenic Vibrio cholerae in rectal swab samples
| Autor: | Orapim Puiprom, Pathom Sawanpanyalert, Takeshi Honda, Siriporn Chantaroj, Yasuhiko Suzuki, Kazuhisa Okada, Tooru Taniguchi, Amonrattana Roobthaisong |
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| Rok vydání: | 2010 |
| Předmět: |
Microbiology (medical)
Cholera Toxin Loop-mediated isothermal amplification Biology medicine.disease_cause Sensitivity and Specificity Microbiology law.invention Bacterial Proteins Cholera Vibrionaceae law medicine Humans Vibrio cholerae Polymerase chain reaction Detection limit Clinical Laboratory Techniques Rectum General Medicine biology.organism_classification medicine.disease Molecular biology genomic DNA Infectious Diseases Carrier State Nucleic Acid Amplification Techniques Bacteria |
| Zdroj: | Diagnostic Microbiology and Infectious Disease. 66:135-139 |
| ISSN: | 0732-8893 |
| DOI: | 10.1016/j.diagmicrobio.2009.09.004 |
| Popis: | Loop-mediated isothermal amplification (LAMP) method was designed for clinical diagnosis of Vibrio cholerae carrying the ctxA gene. The detection limits of the method were 5 fg of purified genomic DNA/reaction and 0.54 CFU/reaction. The method was applied to rectal swab samples from cholera patients and healthy volunteers (19 subjects each) and yielded the same results as the "gold standard" culture method, while the polymerase chain reaction-based method failed to detect V. cholerae in 8 of the positive samples. Direct application of this LAMP method without precultivation enabled the rapid detection of 5 asymptomatic carriers from rectal swabs of 21 household contacts of cholera patients. This LAMP method could be a sensitive, specific, inexpensive, and rapid detection tool for V. cholerae carrying the ctxA gene in the clinical laboratory and in the field. |
| Databáze: | OpenAIRE |
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