Multiplex PCR To Identify Macrolide Resistance Determinants in Mannheimia haemolytica and Pasteurella multocida
| Autor: | Ralf Warrass, Stephen Douthwaite, Puneet Jaju, Simon Rose, Benoit Desmolaize, Cornelia Wilhelm |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Pasteurella multocida
medicine.drug_class Biology Microbiology Macrolide Antibiotics chemistry.chemical_compound 23S ribosomal RNA Mechanisms of Resistance Multiplex polymerase chain reaction Drug Resistance Bacterial medicine Pharmacology (medical) Tulathromycin Tilmicosin Lincosamides Mannheimia haemolytica Pharmacology Streptogramin B biology.organism_classification Virology Anti-Bacterial Agents Infectious Diseases chemistry Macrolides Multiplex Polymerase Chain Reaction |
| Zdroj: | Rose, S, Desmolaize, B, Jaju, P, Wilhelm, C, Warrass, R & Douthwaite, S R 2012, ' Multiplex PCR To Identify Macrolide Resistance Determinants in Mannheimia haemolytica and Pasteurella multocida ', Antimicrobial Agents and Chemotherapy, vol. 56, no. 7, pp. 3664-9 . https://doi.org/10.1128/AAC.00266-12 |
| DOI: | 10.1128/AAC.00266-12 |
| Popis: | The bacterial pathogens Mannheimia haemolytica and Pasteurella multocida are major etiological agents in respiratory tract infections of cattle. Although these infections can generally be successfully treated with veterinary macrolide antibiotics, a few recent isolates have shown resistance to these drugs. Macrolide resistance in members of the family Pasteurellaceae is conferred by combinations of at least three genes: erm (42), which encodes a monomethyltransferase and confers a type I MLS B (macrolide, lincosamide, and streptogramin B) phenotype; msr (E), which encodes a macrolide efflux pump; and mph (E), which encodes a macrolide-inactivating phosphotransferase. Here, we describe a multiplex PCR assay that detects the presence of erm (42), msr (E), and mph (E) and differentiates between these genes. In addition, the assay distinguishes P. multocida from M. haemolytica by amplifying distinctive fragments of the 23S rRNA ( rrl ) genes. One rrl fragment acts as a general indicator of gammaproteobacterial species and confirms whether the PCR assay has functioned as intended on strains that are negative for erm (42), msr (E), and mph (E). The multiplex system has been tested on more than 40 selected isolates of P. multocida and M. haemolytica and correlated with MICs for the veterinary macrolides tulathromycin and tilmicosin, and the newer compounds gamithromycin and tildipirosin. The multiplex PCR system gives a rapid and robustly accurate determination of macrolide resistance genotypes and bacterial genus, matching results from microbiological methods and whole-genome sequencing. |
| Databáze: | OpenAIRE |
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