Calprotectin instability may lead to undertreatment in children with IBD
| Autor: | Anneke C. Muller Kobold, Lucie Wagenmakers, Sjoukje-Marije Haisma, Patrick F. van Rheenen |
|---|---|
| Přispěvatelé: | Center for Liver, Digestive and Metabolic Diseases (CLDM), Guided Treatment in Optimal Selected Cancer Patients (GUTS) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Stool sample Short Report gastroenterology Gastroenterology Inflammatory bowel disease Specimen Handling Feces fluids and secretions Internal medicine medicine Humans Lead (electronics) Child business.industry Protein Stability Temperature medicine.disease Inflammatory Bowel Diseases Faecal calprotectin monitoring Pediatrics Perinatology and Child Health Treatment decision making Calprotectin business Leukocyte L1 Antigen Complex Biomarkers |
| Zdroj: | Archives of Disease in Childhood Archives of Disease in Childhood, 105(10), 996-998. BMJ PUBLISHING GROUP |
| ISSN: | 1468-2044 0003-9888 |
| Popis: | BackgroundTreatment decisions in children with inflammatory bowel disease (IBD) are increasingly based on longitudinal tracking of faecal calprotectin concentrations, but there is little known about the stability of this protein in stool.MethodsWe stored aliquots of homogenised stool at room temperature and at 4°C, and measured the calprotectin concentration for 6 consecutive days with three different assays. In addition, we assessed calprotectin stability in assay-specific extraction buffers kept at room temperature.ResultsAfter 6 days of storage at room temperature, mean percentage change from baseline calprotectin concentrations in stool and extraction buffer was 35% and 46%, respectively. The stability of calprotectin was significantly better preserved in samples stored at 4°C (p=0.0066 and 0.0011, respectively).ConclusionsCalprotectin is not stable at room temperature. Children with IBD and their caretakers may be falsely reassured by low calprotectin values. The best advisable standard for preanalytical calprotectin handling is refrigeration of the stool sample until delivery at the hospital laboratory. |
| Databáze: | OpenAIRE |
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