LAB-1 antagonizes the Aurora B kinase in C. elegans

Autor: Jill M. Schumacher, Sophie Zaaijer, Sarit Smolikov, Monica P. Colaiácovo, Yanjie Gu, Carlos Egydio de Carvalho
Rok vydání: 2008
Předmět:
Embryo
Nonmammalian
Chromosomal Proteins
Non-Histone
Molecular Sequence Data
Aurora B kinase
Fluorescent Antibody Technique
Mitosis
Cell Cycle Proteins
Chromatids
Protein Serine-Threonine Kinases
Biology
Chromosome segregation
Aurora Kinases
Chromosome Segregation
Centromere
Genetics
Animals
Aurora Kinase B
Sister chromatids
Amino Acid Sequence
RNA
Messenger
Meiotic Prophase I
RNA
Small Interfering
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Adenosine Triphosphatases
Sequence Homology
Amino Acid
Cohesin
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Regulation
Developmental
DNA-Binding Proteins
Meiosis
Meiotic sister chromatid cohesion
Immunoglobulin G
Multiprotein Complexes
biological phenomena
cell phenomena
and immunity
RNA
Helminth
Separase
Sister Chromatid Exchange
Research Paper
Developmental Biology
Zdroj: Genes & Development. 22:2869-2885
ISSN: 1549-5477
0890-9369
DOI: 10.1101/gad.1691208
Popis: The Shugoshin/Aurora circuitry that controls the timely release of cohesins from sister chromatids in meiosis and mitosis is widely conserved among eukaryotes, although little is known about its function in organisms whose chromosomes lack a localized centromere. Here we show that Caenorhabditis elegans chromosomes rely on an alternative mechanism to protect meiotic cohesin that is shugoshin-independent and instead involves the activity of a new chromosome-associated protein named LAB-1 (Long Arm of the Bivalent). LAB-1 preserves meiotic sister chromatid cohesion by restricting the localization of the C. elegans Aurora B kinase, AIR-2, to the interface between homologs via the activity of the PP1/Glc7 phosphatase GSP-2. The localization of LAB-1 to chromosomes of dividing embryos and the suppression of mitotic-specific defects in air-2 mutant embryos with reduced LAB-1 activity support a global role of LAB-1 in antagonizing AIR-2 in both meiosis and mitosis. Although the localization of a GFP fusion and the analysis of mutants and RNAi-mediated knockdowns downplay a role for the C. elegans shugoshin protein in cohesin protection, shugoshin nevertheless helps to ensure the high fidelity of chromosome segregation at metaphase I. We propose that, in C. elegans, a LAB-1-mediated mechanism evolved to offset the challenges of providing protection against separase activity throughout a larger chromosome area.
Databáze: OpenAIRE
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