West Nile Virus Seroprevalence in the Greek Population in 2013: A Nationwide Cross-Sectional Survey
| Autor: | Spyros Pournaras, Jenny Kremastinou, Antonios Katsioulis, Persefoni Tserkezou, Malwest, Anna Papa, Andriani Marka, Agoritsa Baka, Christos Hadjichristodoulou, Athanasios Tsakris, Nikos T. Papadopoulos, Anna Psaroulaki, Maria Mavrouli, Zissis Mamuris, Charalambos Billinis |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
medicine.medical_specialty Adolescent Cross-sectional study Science Antibodies Viral Serology Young Adult Plaque reduction neutralization test Seroepidemiologic Studies Epidemiology Medicine Seroprevalence Humans Child Aged Aged 80 and over Multidisciplinary biology Geography Greece business.industry Infant Newborn Outbreak Infant Middle Aged Virology 3. Good health Cross-Sectional Studies Immunoglobulin M Child Preschool Immunoglobulin G Population Surveillance biology.protein business West Nile virus West Nile Fever Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 10, Iss 11, p e0143803 (2015) |
| ISSN: | 1932-6203 |
| Popis: | Cases of West Nile Virus (WNV) disease were recorded for three consecutive years in Greece following the year 2010 outbreak. A cross-sectional serologic survey was conducted to estimate the WNV seroprevalence and assess the ratio of infection to neuroinvasive disease. A stratified left-over sampling methodology was used including age and residence strata. A total of 3,962 serum samples was collected and tested for WNV Immunoglobulin G (IgG) antibodies by Enzyme-Linked Immunosorbent Assay (ELISA). All positive samples were further tested by Plaque Reduction Neutralization Test (PRNT) and WNV Immunoglobulin M (IgM) antibodies. WNV IgG antibodies were detected in 82 samples and 61 were also positive in PRNT representing a weighted seroprevalence of 2.1% (95% C.I.: 1.7-2.6) and 1.5% (95% C.I.: 1.2-2.0), respectively. Multivariable analysis showed that seroprevalence was associated with age and residence. The overall ratio of neuroinvasive disease to infected persons was estimated at 1:376 (95% C.I.: 1:421-1:338), while the elderly people had the highest ratio. This nationwide study provided valuable data regarding the epidemiology of WNV in Greece based on the fact that elderly people have higher risk of being both infected and having severe disease. |
| Databáze: | OpenAIRE |
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