IL-6 regulated stress-induced Rex-1 expression in stem cells from human exfoliated deciduous teeth
| Autor: | Prasit Pavasant, Thanaphum Osathanon, S Taebunpakul, Nattanan Govitvattana |
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| Rok vydání: | 2012 |
| Předmět: |
Neurogenesis
Immunocytochemistry Cell Culture Techniques Kruppel-Like Transcription Factors Biology Stem cell marker Mechanotransduction Cellular Calcification Physiologic Downregulation and upregulation Osteogenesis Deciduous teeth medicine Humans Gene Silencing RNA Small Interfering Tooth Deciduous Neutralizing antibody Interleukin 6 General Dentistry Janus Kinases Adipogenesis Interleukin-6 Cell Differentiation Mesenchymal Stem Cells Zinc Fingers Molecular biology Biomechanical Phenomena Up-Regulation medicine.anatomical_structure Otorhinolaryngology biology.protein Stress Mechanical Signal transduction Stem cell |
| Zdroj: | Oral Diseases. 19:673-682 |
| ISSN: | 1354-523X |
| Popis: | Objective To investigate the effect of mechanical stress on stem cells from human exfoliated deciduous teeth (SHED). Methods Cells were stimulated with mechanical stress ranging from 0 to 2.5 g cm−2 for 2 h in serum-free condition. The expression of stem cell markers was examined by polymerase chain reaction and immunocytochemistry. The molecular mechanisms of these effects were investigated by means of inhibitors and siRNA. Results Mechanical stress induced Rex-1 expression in a force-dependent manner. The upregulation of interleukin-6 (IL-6) was also observed. Application of neutralizing antibody against IL-6 and IL-6 siRNA could attenuate the mechanical stress-induced Rex-1 expression. In addition, the increase in mRNA levels of Rex-1 was observed after treating SHED with an exogenous IL-6. This inductive effect was attenuated by JAK inhibitor, suggesting the involvement of JAK signaling pathway. Conclusion These results suggest the role of mechanical stress in the regulation of stemness via IL-6–Rex-1 interaction. |
| Databáze: | OpenAIRE |
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