Dexamethasone Influences Human Clock Gene Expression in Bronchial Epithelium and Peripheral Blood Mononuclear Cellsin vitro

Autor: Hiroshi Takane, Naoto Burioka, Eiji Shimizu, Miyako Takata, Hisashi Suyama, Shigehiro Ohdo, Yoko Okano, Yasushi Fukuoka, Masahiro Endo, Masanori Miyata
Rok vydání: 2005
Předmět:
Zdroj: Chronobiology International. 22:585-590
ISSN: 1525-6073
0742-0528
DOI: 10.1081/cbi-200062416
Popis: We determined whether human peripheral blood mononuclear cells (PBMCs) could be used to analyze clock genes by studying their mRNA expressions in human bronchial epithelium (BEAS-2B) and PBMCs following stimulation by the glucocorticoid homologue dexamethasone (DEX) in vitro. PBMCs were obtained at 10:00 h from two diurnally active (approximately 07:00 to 23:00 h) healthy volunteers and were evaluated for hPer1 mRNA expression following DEX stimulation in vitro using real time-PCR analysis. DEX stimulation of human BEAS-2B cells and PBMCs in vitro led to a remarkable increase of hPer1 mRNA. The glucocorticoid rapidly affected the expression of hPer1 mRNA in PBMCs, suggesting that human PBMCs may be a useful surrogate marker for the investigation of drug effects on clock genes.
Databáze: OpenAIRE
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