Dexamethasone Influences Human Clock Gene Expression in Bronchial Epithelium and Peripheral Blood Mononuclear Cellsin vitro
Autor: | Hiroshi Takane, Naoto Burioka, Eiji Shimizu, Miyako Takata, Hisashi Suyama, Shigehiro Ohdo, Yoko Okano, Yasushi Fukuoka, Masahiro Endo, Masanori Miyata |
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Rok vydání: | 2005 |
Předmět: |
Adult
Male medicine.medical_specialty Physiology Bronchi Cell Cycle Proteins Stimulation Respiratory Mucosa Biology Peripheral blood mononuclear cell Dexamethasone Cell Line Physiology (medical) Internal medicine medicine Humans Glucocorticoids Messenger RNA Nuclear Proteins hemic and immune systems Period Circadian Proteins Molecular biology In vitro Circadian Rhythm Endocrinology Gene Expression Regulation Cell culture Leukocytes Mononuclear hormones hormone substitutes and hormone antagonists Glucocorticoid medicine.drug PER1 |
Zdroj: | Chronobiology International. 22:585-590 |
ISSN: | 1525-6073 0742-0528 |
DOI: | 10.1081/cbi-200062416 |
Popis: | We determined whether human peripheral blood mononuclear cells (PBMCs) could be used to analyze clock genes by studying their mRNA expressions in human bronchial epithelium (BEAS-2B) and PBMCs following stimulation by the glucocorticoid homologue dexamethasone (DEX) in vitro. PBMCs were obtained at 10:00 h from two diurnally active (approximately 07:00 to 23:00 h) healthy volunteers and were evaluated for hPer1 mRNA expression following DEX stimulation in vitro using real time-PCR analysis. DEX stimulation of human BEAS-2B cells and PBMCs in vitro led to a remarkable increase of hPer1 mRNA. The glucocorticoid rapidly affected the expression of hPer1 mRNA in PBMCs, suggesting that human PBMCs may be a useful surrogate marker for the investigation of drug effects on clock genes. |
Databáze: | OpenAIRE |
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