Community Analysis of Root- and Tuber-Associated Bacteria in Field-Grown Potato Plants Harboring Different Resistance Levels against Common Scab
| Autor: | Nobutaka Someya, Akira Kobayashi, Yuki Ohdaira Kobayashi, Seishi Ikeda |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Population Soil Science Plant Science Plant disease resistance Real-Time Polymerase Chain Reaction community analysis DNA Ribosomal Plant Roots Actinobacteria Streptomyces turgidiscabies Rhizobium etli RNA Ribosomal 16S Botany Cluster Analysis common scab education Phylogeny Soil Microbiology Ecology Evolution Behavior and Systematics Disease Resistance Plant Diseases Solanum tuberosum Rhizosphere education.field_of_study biology Common scab fungi food and beverages Sequence Analysis DNA Articles General Medicine biology.organism_classification Biota Plant Tubers potato Rhizobium 16S rRNA gene rhizosphere |
| Zdroj: | Microbes and Environments |
| ISSN: | 1347-4405 1342-6311 |
| DOI: | 10.1264/jsme2.me15109 |
| Popis: | Eight genotypes of potato plants with different resistance levels against common scab were grown in a field infested with Streptomyces turgidiscabies. DNA was extracted from the roots, tubers, and rhizosphere soils of each of the eight genotypes at the flowering stage, and the quantity of S. turgidiscabies genomic DNA was assessed by real-time PCR using a TaqMan probe. The results obtained showed that the different potato genotypes had significant impacts on the population levels of S. turgidiscabies between resistant and susceptible genotypes in the tubers, but not in the roots or rhizosphere soils. Clone analyses of 16S rRNA gene libraries from the eight potato genotypes identified three phyla (Proteobacteria, Firmicutes, and Actinobacteria) as dominant taxa in root and tuber clone libraries, while a clustering analysis identified 391 operational taxonomic units (OTUs) at the species level. Eleven OTUs closely related to Aquicella siphonis, Arthrobacter nicotinovorans, Streptomyces rishiriensis, Rhodococcus baikonurensis, Rhizobium radiobacter, Rhizobium etli, Phyllobacterium myrsinacearum, Paenibacillus pabuli, Paenibacillus alginolyticus, and Bacillus halmapalus were detected in the root or tuber libraries of all the potato genotypes examined. Furthermore, an abundance of OTUs related to Aquicella and Rhodococcus was observed in the rhizospheres of resistant and susceptible potato genotypes, respectively. Based on this ecological information, an efficient survey may be conducted for biological agents from the potato rhizosphere. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|