Simultaneous structural and elemental nano-imaging of human brain tissue
| Autor: | Kay L. Double, Sian Genoud, Dominic J. Hare, Si Chen, Junjing Deng, Benjamin G. Trist, Michael W. M. Jones |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Elemental composition Chemistry General Chemistry Human brain Characterization (materials science) 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Neuromelanin Fluorescence microscope medicine Biophysics Correlative imaging Structural imaging 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Chemical Science |
| ISSN: | 2041-6539 2041-6520 |
| Popis: | Examining chemical and structural characteristics of micro-features in complex tissue matrices is essential for understanding biological systems. Advances in multimodal chemical and structural imaging using synchrotron radiation have overcome many issues in correlative imaging, enabling the characterization of distinct microfeatures at nanoscale resolution in ex vivo tissues. We present a nanoscale imaging method that pairs X-ray ptychography and X-ray fluorescence microscopy (XFM) to simultaneously examine structural features and quantify elemental content of microfeatures in complex ex vivo tissues. We examined the neuropathological microfeatures Lewy bodies, aggregations of superoxide dismutase 1 (SOD1) and neuromelanin in human post-mortem Parkinson's disease tissue. Although biometals play essential roles in normal neuronal biochemistry, their dyshomeostasis is implicated in Parkinson's disease aetiology. Here we show that Lewy bodies and SOD1 aggregates have distinct elemental fingerprints yet are similar in structure, whilst neuromelanin exhibits different elemental composition and a distinct, disordered structure. The unique approach we describe is applicable to the structural and chemical characterization of a wide range of complex biological tissues at previously unprecedented levels of detail. Structural and chemical characterisation of microfeatures in unadulterated Parkinson's disease brain tissue using synchrotron nanoscale XFM and ptychography. |
| Databáze: | OpenAIRE |
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