The Scf/Kit pathway implements self-organized epithelial patterning
| Autor: | Charlotte Rulquin, Laurent Kodjabachian, Virginie Thomé, Raphaël Clément, Alexandre Chuyen, Fabrice Daian, Andrea Pasini |
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| Přispěvatelé: | Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS), Turing Centre for Living Systems, Marseille, France, Turing Centre for Living Systems [Marseille] (TCLS) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
contact inhibition of locomotion
Embryo Nonmammalian actin cytoskeleton Xenopus [SDV]Life Sciences [q-bio] Population self-organisation Video microscopy Xenopus Proteins cell motility cell-cell repulsion Cell junction General Biochemistry Genetics and Molecular Biology Receptor tyrosine kinase Actin-Related Protein 2-3 Complex 03 medical and health sciences Xenopus laevis 0302 clinical medicine pattern formation Animals Cilia education Molecular Biology 030304 developmental biology 0303 health sciences education.field_of_study Stem Cell Factor biology Actin remodeling Gene Expression Regulation Developmental Cell Biology biology.organism_classification Actin cytoskeleton Embryonic stem cell Actins Scf/Kit Cell biology Proto-Oncogene Proteins c-kit adhesion Intercellular Junctions ciliated epithelium Epidermal Cells biology.protein Female 030217 neurology & neurosurgery Developmental Biology Signal Transduction |
| Zdroj: | Developmental Cell Developmental Cell, Elsevier, 2021, 56 (6), pp.795-810.e7. ⟨10.1016/j.devcel.2021.02.026⟩ Developmental Cell, 2021, 56 (6), pp.795-810.e7. ⟨10.1016/j.devcel.2021.02.026⟩ |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2021.02.026⟩ |
| Popis: | International audience; How individual cell behaviours lead to the emergence of global patterns is poorly understood. In the Xenopus embryonic epidermis, multiciliated cells (MCCs) are born in a random pattern within an inner mesenchymal layer, and subsequently intercalate at regular intervals into an outer epithelial layer. Using both experiments and mathematical modelling, we show that this transition from random to ordered distribution relies on mutual repulsion among motile immature MCCs, and affinity towards outer-layer intercellular junctions. Consistently, Arp2/3-mediated actin remodelling is required for MCC pattern emergence. Using multiple functional approaches, we show that the Kit tyrosine kinase receptor, expressed in MCCs, and its ligand Scf, expressed in outer-layer cells, are both required for regular MCC distribution. Membrane-associated Scf behaves as a potent adhesive cue for MCCs, while its soluble form promotes their mutual repulsion. On the other hand, Kit expression is sufficient to confer order to a disordered heterologous cell population. Our work reveals how a single signalling system can implement self-organised large-scale patterning. |
| Databáze: | OpenAIRE |
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