Confirmed Previous Infection With Chlamydia pneumoniae (TWAR) and Its Presence in Early Coronary Atherosclerosis
| Autor: | J. Thomas Grayston, Lee Ann Campbell, John P. Middaugh, San-Pin Wang, John C. Finley, Michael H. Davidson, Cho Chou Kuo, William P. Newman |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent Coronary Artery Disease Polymerase Chain Reaction Serology Risk Factors Cause of Death Physiology (medical) medicine Humans Chlamydiaceae Risk factor Coronary atherosclerosis Chlamydia biology business.industry Myocardium Heart Chlamydia Infections Chlamydophila pneumoniae Middle Aged medicine.disease biology.organism_classification Antibodies Bacterial Coronary Vessels Immunohistochemistry Immunoglobulin A Atheroma Immunoglobulin M Immunoglobulin G Chlamydiales Indians North American biology.protein Female Autopsy Antibody Cardiology and Cardiovascular Medicine business Alaska |
| Zdroj: | Circulation. 98:628-633 |
| ISSN: | 1524-4539 0009-7322 |
| DOI: | 10.1161/01.cir.98.7.628 |
| Popis: | Background — Chlamydia pneumoniae has been identified in coronary atheroma, but concomitant serum antibody titers have been inconsistently positive and unavailable before the detection of early or advanced atherosclerotic lesions. Methods and Results —This retrospective investigation was performed on premortem serum specimens and autopsy tissue from 60 indigenous Alaska Natives at low risk for coronary heart disease, selected by the potential availability of their stored specimens. Serum specimens were drawn a mean of 8.8 years (range, 0.7 to 26.2 years) before death, which occurred at a mean age of 34.1 years (range, 15 to 57 years), primarily from noncardiovascular causes (97%). Coronary artery tissues were independently examined histologically and, for C pneumoniae organism and DNA, by immunocytochemistry (ICC) and polymerase chain reaction (PCR) with species-specific monoclonal antibody and primers. Microimmunofluorescence detected species-specific IgG, IgA, and IgM antibody in stored serum. C pneumoniae , frequently within macrophage foam cells, was identified in coronary fibrolipid atheroma (raised lesions, Stary types II through V) in 15 subjects (25%) and early flat lesions in 7 (11%) either by PCR (14, 23%) or ICC (20, 33%). The OR for C pneumoniae in raised atheroma after a level of IgG antibody ≥1:256 >8 years earlier was 6.1 (95% CI, 1.1 to 36.6) and for all coronary tissues after adjustment for multiple potential confounding variables, including tobacco exposure, was 9.4 (95% CI, 2.6 to 33.8). Conclusions —Serological evidence for C pneumoniae infection frequently precedes both the earliest and more advanced lesions of coronary atherosclerosis that harbor this intracellular pathogen, suggesting a chronic infection and developmental role in coronary heart disease. |
| Databáze: | OpenAIRE |
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