Permeabilized myocardial fibers as model to detect mitochondrial dysfunction during sepsis and melatonin effects without disruption of mitochondrial network
| Autor: | María E. Díaz-Casado, Luis C. López, Germaine Escames, Beatriz Fernández-Gil, Carolina Doerrier, Huayqui Volt, Jose A. García, Darío Acuña-Castroviejo, Marta Luna-Sánchez |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Bioenergetics Oxidative phosphorylation Biology Antioxidants Mitochondria Heart Oxidative Phosphorylation Electron Transport Melatonin Sepsis Electron Transport Complex III 03 medical and health sciences Myofibrils medicine Animals Respiratory system Molecular Biology Myocardium Cell Biology medicine.disease Cell biology Mice Inbred C57BL Disease Models Animal 030104 developmental biology Biochemistry Coenzyme Q – cytochrome c reductase Molecular Medicine Myofibril Intracellular medicine.drug |
| Zdroj: | Mitochondrion. 27:56-63 |
| ISSN: | 1567-7249 |
| DOI: | 10.1016/j.mito.2015.12.010 |
| Popis: | Analysis of mitochondrial function is crucial to understand their involvement in a given disease. High-resolution respirometry of permeabilized myocardial fibers in septic mice allows the evaluation of the bioenergetic system, maintaining mitochondrial ultrastructure and intracellular interactions, which are critical for an adequate functionality. OXPHOS and electron transport system (ETS) capacities were assessed using different substrate combinations. Our findings show a severe septic-dependent impairment in OXPHOS and ETS capacities with mitochondrial uncoupling at early and late phases of sepsis. Moreover, sepsis triggers complex III (CIII)-linked alterations in supercomplexes structure, and loss of mitochondrial density. In these conditions, melatonin administration to septic mice prevented sepsis-dependent mitochondrial injury in mitochondrial respiration. Likewise, melatonin improved cytochrome b content and ameliorated the assembly of CIII in supercomplexes. These results support the use of permeabilized fibers to identify properly the respiratory deficits and specific melatonin effects in sepsis. |
| Databáze: | OpenAIRE |
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