Bean yellow mosaic virus infecting broad bean in the green belt of Córdoba, Argentina
| Autor: | Nacira Muñoz, Claudia Nome, Evangelina Beatriz Arguello Caro, Humberto Julio Debat, Pablo Gastón Reyna, Patricia Rodriguez Pardina, Andrés V. Luque |
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| Rok vydání: | 2019 |
| Předmět: |
Indirect elisa
Veterinary medicine biology Virus Mosaico Amarillo del Frijol Cinturón Verde de Córdoba Inoculation Potyvirus Total rna Argentina Next Generation Sequencing food and beverages Pulse crop Growing season Secuenciación de Alto Rendimiento Bean yellow mosaic virus Bean Yellow Mosaic Virus biology.organism_classification Virus Virions Vicia faba High-Throughput Sequencing Virus Particles Virión Vicia Faba Cordoba (Argentina) |
| Zdroj: | BioRxiv : the preprint server for biology. (April 11, 2019) INTA Digital (INTA) Instituto Nacional de Tecnología Agropecuaria instacron:INTA |
| Popis: | Broad bean (Vicia faba L) is the fourth most important pulse crop in the world. In Argentina, broad bean production was of 1,841 hectares and 16,500 tons during the 2017 growing season. Broad bean is commonly used in rotations; especially by farmers located in “green belts” that are peri-urban areas surrounding large cities that include horticultural family farms. Plants showing marked foliar mosaic symptoms, typical of viral infection, were collected during the 2015 growing season in the green belt of Córdoba city, Argentina. Preparations of symptomatic tissues were mechanically inoculated onto healthy broad bean plants in the greenhouse, which developed symptoms similar to those observed in the field. In addition, symptomatic samples were positive when tested by indirect ELISA with the anti-potyvirus group monoclonal antibody. Further, flexuous filamentous particles typical of potyviruses were observed under the electronic microscope on dip preparations. Lastly, total RNA was extracted from a symptomatic leaf and high-throughput sequenced, which allowed the assembly of a single virus sequence corresponding to a new highly divergent strain of Bean yellow mosaic virus (BYMV). Phylogenetic insights clustered this Argentinean broad bean isolate (BYMV-ARGbb) within group IX of BYMV. Given the economical importance of this virus and its associated disease, the results presented here are a pivotal first step oriented to explore the eventual incidence and epidemiological parameters of BYMV in broad bean in Argentina. Instituto de Patología Vegetal Fil: Rodriguez Pardina, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Reyna, Pablo Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Reyna, Pablo Gastón. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Muñoz, Nacira Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Muñoz, Nacira Belén. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Exactas Físicas y Naturales (FCEFyN).Cátedra de Fisiología Vegetal; Argentina Fil: Arguello Caro, Evangelina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Arguello Caro, Evangelina Beatriz. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias (FCA). Cátedra de Zoología Agrícola,; Argentina Fil: Luque, Andres Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Luque, Andres Vicente. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina |
| Databáze: | OpenAIRE |
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