Expression of the AMACO (VWA2 protein) ortholog in zebrafish
| Autor: | Kristina Røkenes Karlsen, Raimund Wagener, Mats Paulsson, Jan M. Gebauer, Wolfram F. Neiss |
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| Rok vydání: | 2010 |
| Předmět: |
Gills
animal structures EGF-like domain Molecular Sequence Data Gene Expression Mice Genetics medicine Consensus sequence Animals Amino Acid Sequence Cloning Molecular Muscle Skeletal Molecular Biology Gene Zebrafish Conserved Sequence In Situ Hybridization DNA Primers Skin Extracellular Matrix Proteins Base Sequence biology Reverse Transcriptase Polymerase Chain Reaction Computational Biology Sequence Analysis DNA Zebrafish Proteins Protein superfamily biology.organism_classification Immunohistochemistry Molecular biology Protein Structure Tertiary Pronephros Somite medicine.anatomical_structure VWA2 Developmental Biology |
| Zdroj: | Gene Expression Patterns. 10:53-59 |
| ISSN: | 1567-133X |
| Popis: | AMACO is a basement membrane associated protein that belongs to the VWA domain-containing protein superfamily. In addition to three VWA domains it contains two EGF-like domains, a cysteine-rich domain and a unique domain. Mouse AMACO has been partially characterized, but its function remains unknown. The zebrafish genome contains a single AMACO ortholog gene on chromosome 12. The domain structure is completely conserved between zebrafish and mouse and the first EGF-like domain, carrying a rare O-glucosylation and O-fucosylation consensus sequence, has the highest identity at the protein level. RT-PCR shows strongest AMACO expression during development, starting at the 5 somite stage. An antibody specific for zebrafish AMACO detected expression mainly in myosepta but also in skin, pronephros, pituitary gland, otic capsule and gills. In situ hybridization revealed that the muscle precursor cells of the somites express the protein that is laid down in the myosepta. |
| Databáze: | OpenAIRE |
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