Butyrate Enhances the In Vitro Anti-SRBC (Sheep Red Blood Cell) Antibody Responses in Murine Splenocytes
| Autor: | Masami Fujiwara, Itaru Yamamoto, Yumiko Kishiro, Kumiko Ueda |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Interleukin 2
medicine.medical_specialty Erythrocytes Hemolytic Plaque Technique Butyrate In Vitro Techniques Butyric acid Mice chemistry.chemical_compound Internal medicine medicine Animals Pharmacology Mice Inbred BALB C Sheep biology Short-chain fatty acid Interleukin Butyrates Endocrinology chemistry Concanavalin A Cell culture Antibody Formation biology.protein Interleukin-2 Female Antibody Spleen Interleukin-1 medicine.drug |
| Zdroj: | Japanese Journal of Pharmacology. 66:369-376 |
| ISSN: | 0021-5198 |
| Popis: | Butyrate at concentrations of 200-600 microM markedly enhanced the in vitro antibody productions against sheep red blood cells (SRBC) in murine splenocytes. However, other saturated short-chain fatty acids, including acetate, propionate and valeric acid, and 4-carbon compounds such as butanol, acetoacetate and beta- and gamma-hydroxybutyrate had no such effects. The presence of butyrate in the early phase of the cell culture was crucial for enhancement of the response. Butyrate also augmented the antibody production in T-cell-depleted splenocytes supplemented with the culture supernatant of concanavalin A (Con A)-stimulated lymphocytes. Interleukin (IL)-2 secreted from splenocytes in response to SRBC was increased by adding butyrate to the culture, but IL-1 secretion was not affected. On the other hand, Con A or lipopolysaccharide-stimulated proliferation of splenocytes was partly depressed by the addition of butyrate, while Con A-induced IL-2 production was not effected. These findings suggest that butyrate may act on T and B cells to promote their differentiation during the process of antibody production. |
| Databáze: | OpenAIRE |
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