High-Throughput MicroRNA Profiling of Vitreoretinal Lymphoma: Vitreous and Serum MicroRNA Profiles Distinct from Uveitis
| Autor: | Kinya Tsubota, Akitomo Narimatsu, Yoshihiko Usui, Masahiko Kuroda, Masakatsu Takanashi, Naoyuki Yamakawa, Hiroyuki Shimizu, Masaki Asakage, Hiroshi Goto, Teruumi Minezaki, Kazuhiko Umazume, Naoya Nezu |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
lcsh:Medicine Disease Article Pathogenesis 03 medical and health sciences 0302 clinical medicine microRNA medicine B cell business.industry lcsh:R Interleukin vitreoretinal lymphoma General Medicine medicine.disease Lymphoma 030104 developmental biology medicine.anatomical_structure machine learning 030220 oncology & carcinogenesis Cancer research uveitis Epiretinal membrane business Uveitis |
| Zdroj: | Journal of Clinical Medicine, Vol 9, Iss 1844, p 1844 (2020) Journal of Clinical Medicine Volume 9 Issue 6 |
| ISSN: | 2077-0383 |
| Popis: | Purpose: Vitreoretinal lymphoma (VRL) is a non-Hodgkin lymphoma of the diffuse large B cell type (DLBCL), which is an aggressive cancer causing central nervous system related mortality. The pathogenesis of VRL is largely unknown. The role of microRNAs (miRNAs) has recently acquired remarkable importance in the pathogenesis of many diseases including cancers. Furthermore, miRNAs have shown promise as diagnostic and prognostic markers of cancers. In this study, we aimed to identify differentially expressed miRNAs and pathways in the vitreous and serum of patients with VRL and to investigate the pathogenesis of the disease. Materials and Methods: Vitreous and serum samples were obtained from 14 patients with VRL and from controls comprising 40 patients with uveitis, 12 with macular hole, 14 with epiretinal membrane, 12 healthy individuals. The expression levels of 2565 miRNAs in serum and vitreous samples were analyzed. Results: Expression of the miRNAs correlated significantly with the extracellular matrix (ECM) ‒receptor interaction pathway in VRL. Analyses showed that miR-326 was a key driver of B-cell proliferation, and miR-6513-3p could discriminate VRL from uveitis. MiR-1236-3p correlated with vitreous interleukin (IL)-10 concentrations. Machine learning analysis identified miR-361-3p expression as a discriminator between VRL and uveitis. Conclusions: Our findings demonstrate that aberrant microRNA expression in VRL may affect the expression of genes in a variety of cancer-related pathways. The altered serum miRNAs may discriminate VRL from uveitis, and serum miR-6513-3p has the potential to serve as an auxiliary tool for the diagnosis of VRL. |
| Databáze: | OpenAIRE |
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