Identification of α-tocopherol as a bioactive component of Dicranopteris linearis with disrupting property against preformed biofilm of Staphylococcus aureus
| Autor: | Yau Yan Lim, Kuan Shion Ong, Christina Injan Mawang, Azira Muhamad, Sui Mae Lee |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Staphylococcus aureus 030106 microbiology alpha-Tocopherol Ethyl acetate medicine.disease_cause Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences chemistry.chemical_compound medicine Bioassay Humans biology Plant Extracts Broth microdilution Biofilm Biofilm matrix General Medicine biochemical phenomena metabolism and nutrition Staphylococcal Infections biology.organism_classification Antimicrobial Anti-Bacterial Agents Plant Leaves 030104 developmental biology chemistry Dicranopteris linearis Biofilms Ferns Biotechnology |
| Zdroj: | Journal of applied microbiology. 123(5) |
| ISSN: | 1365-2672 |
| Popis: | Aims The potential of Dicranopteris linearis leaves extract and its bioactive components were investigated for the first time for its disrupting ability against Staphylococcus aureus biofilms. Methods and Results The leaves of D. linearis were subjected to sonication-assisted extraction using hexane, dichloromethane, ethyl acetate and methanol. It was found that only the methanol fraction exhibited antimicrobial activity using broth microdilution assay; while all four fractions do not exhibit biofilm inhibition activity against S. aureus ATCC 6538P, S. aureus ATCC 43300, S. aureus ATCC 33591 and S. aureus ATCC 29213 using crystal violet assay. Among the four fractions tested, only the hexane extract showed biofilm disrupting ability, with 60-90% disruption activity at 5 mg ml−1 against all four S. aureus strains tested. Bioassay guided purification of the active fraction has led to the isolation of α-tocopherol. α-tocopherol does not affect the cells within the biofilms but instead affects the biofilm matrix in order to disrupt S. aureus biofilms. Conclusions α-tocopherol was identified to be the bioactive component of D. linearis with disruption activity against S. aureus biofilm matrix. Significance and Impact of the Study The use of α-tocopherol as a biofilm disruptive agent might potentially be useful to treat biofilm-associated infections in the future. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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