Using ITS2 PCR-RFLP to generate molecular markers for authentication of Sophora flavescens Ait
| Autor: | Ya Ming Cheng, Li Chang Lin, Mau Shing Yeh, Tzu Che Lin, Jih Min Sung |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Genetic Markers
China DNA Plant Molecular Sequence Data Restriction Mapping Taiwan Biology Plant Roots law.invention Restriction map Species Specificity law parasitic diseases Glycyrrhiza uralensis Amplified Fragment Length Polymorphism Analysis Internal transcribed spacer Phylogeny Polymerase chain reaction Nutrition and Dietetics Sophora flavescens Base Sequence DNA Restriction Enzymes biology.organism_classification Molecular biology Restriction enzyme Genetic marker DNA Intergenic Restriction fragment length polymorphism Databases Nucleic Acid Sequence Alignment Sophora Agronomy and Crop Science Polymorphism Restriction Fragment Length Drugs Chinese Herbal Food Science Biotechnology |
| Zdroj: | Journal of the Science of Food and Agriculture. 92:892-898 |
| ISSN: | 0022-5142 |
| Popis: | BACKGROUND:DriedrootofSophoraflavescensAit.isamedicinalmaterialoccasionallymisusedoradulteratedbyotherspecies similar in appearance. In this study the internal transcribed spacer (ITS) regions of DNA samples of S. flavescens Ait. collected from different areas of Taiwan were amplified by polymerase chain reaction (PCR) and compared. The effectiveness of using ITS2 PCR restriction fragment length polymorphism (RFLP)-generated markers to differentiate S. flavescens Ait. from possible adulterants was also evaluated. RESULTS: The S.flavescens Ait. samples collected from different areas were extremely low in ITS sequence variability at species level. ITS2 PCR-RFLP coupled with restriction enzymes Sac I, Sac II, Xho Io rPvu I produced specific fragments for all tested variants. ITS2 PCR-RFLP coupled with Sac II was further performed to identify mixtures of DNA extracts of S.flavescens Ait. and Sophoratomentosa L. in various ratios. The developed ITS2 PCR-RFLP markers could detect mixed DNA samples of S.flavescens Ait./S.tomentosa L. up to a ratio of 10:1. CONCLUSION: The present study demonstrates the usefulness of ITS2 PCR-RFLP coupled with pre-selected restriction enzymes forpracticalandaccurateauthenticationofS.flavescensAit.ThetechniqueisalsosuitableforanalysingS.flavescensAit.mixed with other adulterants. c � 2011 Society of Chemical Industry |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text