The effect of Misoprostol, a prostaglandin E1 analog, on apoptosis in ischemia-reperfusion-induced intestinal injury
| Autor: | Seda Vatansever, İsmet Topçu, Ahmet Var, Serap Cilaker, Melek Sakarya, Zuhal Cavus |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Male
Histology Prostaglandin Nitric Oxide Synthase Type II Apoptosis Pharmacology Endothelial NOS chemistry.chemical_compound Ileum Malondialdehyde Medicine Animals Alprostadil Rats Wistar Prostaglandin E1 Misoprostol chemistry.chemical_classification TUNEL assay biology business.industry Superoxide Dismutase Glutathione peroxidase Cell Biology General Medicine Rats Nitric oxide synthase chemistry Reperfusion Injury Immunology biology.protein business Oxidoreductases medicine.drug |
| Popis: | The aim of this study was to investigate whether Misoprostol, a synthetic prostaglandin (PG) E1 analog, has any effect on the prevention of apoptosis in ischemia-reperfusion (I/R)-induced intestinal injury. Thirty adult mate Wistar albino rats were divided into three groups: group I = sham operated+satine; group II = I/R+saline; and group III = I/R+Misoprostol. Misoprostol. (50 mu g/kg/d) was administered as an intragastric meat for 3 days. The terminal ileum was collected for histological and biochemical. investigations. Apoptotic cells were detected by terminal deoxynucteotidyl transferase-mediated dUTP nick end-labetted (TUNEL) reaction. Immunohistochemical. analysis was performed to determine the distribution of inducible nitric oxide synthase (iNOS) and endothelial NOS (eNOS). Samples were also analyzed for matondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px). The number of TUNEL-positive cells was higher in group II when compared to the other two groups (p < 0.05). In group III this value was higher when compared to group I, but lower than group II (p |
| Databáze: | OpenAIRE |
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