A simplified method to calculate telomere length from Southern blot images of terminal restriction fragment lengths
| Autor: | Jayne Gilbert, Lisa F. Lincz, Fiona E. Scorgie, Jennette A. Sakoff, Madhu B. Garg |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male General Biochemistry Genetics and Molecular Biology Restriction fragment 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Image Processing Computer-Assisted Humans Statistical software 030304 developmental biology Southern blot Mathematics Aged 0303 health sciences biology Gold standard (test) Middle Aged Telomere Peripheral blood Healthy Volunteers Blotting Southern Terminal (electronics) biology.protein Female Cancer cell lines Algorithm 030217 neurology & neurosurgery Software Biotechnology |
| Zdroj: | BioTechniques. 68(1) |
| ISSN: | 1940-9818 |
| Popis: | Southern blotting of DNA terminal restriction fragment lengths is the gold standard for measuring mean telomere length. Analysis of the final image is a crucial step in this process, however, current techniques are cumbersome and prone to error. Here we present a simple and accurate method for analyzing telomere smears. Basic 2D gel imaging software was used to automatically subtract background, generate standard curves and calculate net intensity and MW at each position (i) along the telomere smear. Our method required no statistical software or major data manipulation and correctly classified >80% of 18 samples as having short, medium or long telomeres compared with 33–72% using other methods. |
| Databáze: | OpenAIRE |
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