Cellular immunity predominates over humoral immunity after homologous and heterologous mRNA and vector‐based COVID‐19 vaccine regimens in solid organ transplant recipients
| Autor: | Heinrike Wilkens, David Schub, Verena Klemis, Sophie Schneitler, Janine Mihm, Urban Sester, Martina Sester, Tina Schmidt, Matthias C. Reichert |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cellular immunity
COVID-19 Vaccines T cell infectious disease Heterologous Priming (immunology) clinical research/practice Antibodies Viral T cell biology infection and infectious agents ‐ viral vaccine Immunology and Allergy Medicine Humans Pharmacology (medical) RNA Messenger Transplantation Immunity Cellular biology business.industry SARS-CoV-2 Immunogenicity flow cytometry COVID-19 Organ Transplantation Clinical Science Antibodies Neutralizing Transplant Recipients Immunity Humoral Vaccination medicine.anatomical_structure Immunology Humoral immunity biology.protein Original Article Antibody ORIGINAL ARTICLES business |
| Zdroj: | American Journal of Transplantation |
| ISSN: | 1600-6143 1600-6135 |
| Popis: | Knowledge on the immunogenicity of vector‐based and mRNA‐vaccines in solid organ transplant recipients is limited. Therefore, SARS‐CoV‐2–specific T cells and antibodies were analyzed in 40 transplant recipients and 70 controls after homologous or heterologous vaccine‐regimens. Plasmablasts and SARS‐CoV‐2–specific CD4 and CD8 T cells were quantified using flow cytometry. Specific antibodies were analyzed by ELISA and neutralization assay. The two vaccine types differed after the first vaccination, as IgG and neutralizing activity were more pronounced after mRNA priming (p = .0001 each), whereas CD4 and CD8 T cell levels were higher after vector priming (p = .009; p = .0001). All regimens were well tolerated, and SARS‐CoV‐2–specific antibodies and/or T cells after second vaccination were induced in 100% of controls and 70.6% of transplant recipients. Although antibody and T cell levels were lower in patients, heterologous vaccination led to the most pronounced induction of antibodies and CD4 T cells. Plasmablast numbers were significantly higher in controls and correlated with SARS‐CoV‐2–specific IgG‐ and T cell levels. While antibodies were only detected in 35.3% of patients, cellular immunity was more frequently found (64.7%) indicating that assessment of antibodies is insufficient to identify COVID‐19‐vaccine responders. In conclusion, heterologous vaccination seems promising in transplant recipients, and combined analysis of humoral and cellular immunity improves the identification of responders among immunocompromised individuals. Among solid organ transplant recipients, a heterologous COVID‐19 vaccine regimen (adenovirus and mRNA vaccine) compared to homologous regimens (two adenovirus or two mRNA vaccines) induce both antibodies and CD4 T cell responses best and combined analysis of humoral and cellular immunity improves identification of responders. |
| Databáze: | OpenAIRE |
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