Genetic and serological characterization of chrysanthemum stem necrosis virus, a member of the genus Tospovirus
| Autor: | Mitsuru Okuda, Yun-Chi Wang, Shyi-Dong Yeh, Tsung-Chi Chen, Minoru Takeshita, Wan-Chu Chien, Pei-Ru Wu |
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| Rok vydání: | 2014 |
| Předmět: |
Chrysanthemum
Molecular Sequence Data Virus Mice chemistry.chemical_compound Tospovirus Virology RNA polymerase Tobacco Animals Serologic Tests Amino Acid Sequence Movement protein Impatiens necrotic spot virus Plant Diseases Antiserum Mice Inbred BALB C Base Sequence Sequence Homology Amino Acid biology Sequence Analysis RNA Antibodies Monoclonal RNA General Medicine Nucleocapsid Proteins biology.organism_classification Molecular biology chemistry Polyclonal antibodies biology.protein RNA Viral Rabbits |
| Zdroj: | Archives of Virology. 160:529-536 |
| ISSN: | 1432-8798 0304-8608 |
| Popis: | Chrysanthemum stem necrosis virus (CSNV) is a member of a tentative tospovirus species. In this study, the complete genomic sequence of the Japanese CSNV isolate TcCh07A was determined. The L RNA is 8960 nt long and encodes the 331.0-kDa RNA-dependent RNA polymerase. The M RNA is 4828 nt long and encodes the 34.1-kDa movement protein (NSm) and the 127.7-kDa glycoprotein precursor (Gn/Gc). The S RNA is 2949 nt long and encodes the 52.4-kDa silencing suppressor protein (NSs) and the 29.3-kDa nucleocapsid (N) protein. The N protein of CSNV-TcCh07A was purified from virus-infected plant tissues and used for production of a rabbit polyclonal antiserum (RAs) and a monoclonal antibody (MAb). Results of serological tests by indirect ELISA and western blotting using the prepared RAs and MAb and a previously produced RAs against the N protein of tomato spotted wilt virus (TSWV) indicated that CSNV-TcCh07A, TSWV, tomato chlorotic spot virus, groundnut ringspot virus, alstroemeria necrotic streak virus and impatiens necrotic spot virus are serologically related. |
| Databáze: | OpenAIRE |
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