Mixed glycerol and orange peel-based substrate for fed-batch microbial biodiesel production
| Autor: | Maurizio Petruccioli, Silvia Crognale, Eleonora Carota, Alessandro D’Annibale |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Circular economy Oleaginous yeasts Continuous stirred-tank reactor Biotechnology Microbiology Waste treatment Green engineering Sustainable development Microbial biotechnology Biofuel Orange peel waste Biodiesel Fed-batch process Rhodosporidium toruloides Oleaginous yeasts Circular economy Orange (colour) Raw material Microbiology Microbial biotechnology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Biofuel Sustainable development Glycerol Fed-batch process Food science lcsh:Social sciences (General) lcsh:Science (General) Green engineering Biodiesel Multidisciplinary Chemistry Rhodosporidium toruloides Biorefinery Orange peel waste Waste treatment 030104 developmental biology Biodiesel production lcsh:H1-99 030217 neurology & neurosurgery lcsh:Q1-390 Research Article Biotechnology |
| Zdroj: | Heliyon Heliyon, Vol 6, Iss 9, Pp e04801-(2020) |
| ISSN: | 2405-8440 |
| Popis: | The aqueous extraction of orange peel waste (OPW), the byproduct of the juice extraction process generated annually in massive amounts (21 Mton), yields a carbohydrate-rich liquid fraction, termed orange peel extract (OPE). Several studies highlight that the combination of glycerol, a biodiesel byproduct, with carbohydrate mixtures might boost microbial lipid production. This study performed first a shaken flask screening of 15 oleaginous yeast strains based on their growth and lipid-producing abilities on OPE- and glycerol-based media. This screening enabled the selection of R. toruloides NRRL 1091 for the assessment of the process transfer in a stirred tank reactor (STR). This assessment relied, in particular, on either single- and double-stage feeding fed-batch (SSF-FB and DSF-FB, respectively) processes where OPE served as the primary medium and nitrogen-containing glycerol-OPE mixtures as the feeding one. The continuous supply mode at low dilution rates (0.02 and 0.01 h−1 for SSF-FB and DSF-FB, respectively) starting from the end of the exponential growth of the initial batch phase enabled the temporal extension of biomass and lipid production. The SSF-FB and DSF-FB processes attained high biomass and lipid volumetric productions (LVP) and ensured significant lipid accumulation on a dry cell basis (YL/X). The SSF-FB process led to LVP of 20.6 g L−1 after 104 h with volumetric productivity (rL) of 0.20 g L−1 h−1 and YL/X of 0.80; the DSF-FB process yielded LVP, rL and YL/X values equal to 15.92 g L−1, 0.11 g L−1 h−1 and 0.65, respectively. The fatty acid profiles of lipids from both fed-batch processes were not significantly different and resembled that of Jatropha oil, a vastly used feedstock for biodiesel production. These results suggest that OPE constitutes an excellent basis for the fed-batch production of R. toruloides lipids, and this process might afford a further option in OPW-based biorefinery. Biotechnology; Microbiology; Waste Treatment; Green Engineering; Sustainable Development; Microbial Biotechnology; Biofuel; orange peel waste; Biodiesel; fed-batch process; Rhodosporidium toruloides; oleaginous yeasts; Circular economy. |
| Databáze: | OpenAIRE |
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