Ara h 2 and Ara h 6 Have Similar Allergenic Activity and Are Substantially Redundant
| Autor: | Rabab El-Mezayen, Yonghua Zhuang, Qian Wang, Xueni Chen, Stephen C. Dreskin |
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| Rok vydání: | 2012 |
| Předmět: |
Arachis
Immunology medicine.disease_cause Immunoglobulin E Article Allergen Antigen Cell Line Tumor Botany medicine Animals Humans Immunology and Allergy Potency Peanut Hypersensitivity heterocyclic compounds Plant metabolism Polyacrylamide gel electrophoresis Glycoproteins biology Plant Extracts Chemistry food and beverages General Medicine Allergens Antigens Plant biochemical phenomena metabolism and nutrition Molecular biology Basophils Rats carbohydrates (lipids) Cell culture biology.protein Electrophoresis Polyacrylamide Gel lipids (amino acids peptides and proteins) Plant immunology 2S Albumins Plant Protein Binding |
| Zdroj: | International Archives of Allergy and Immunology. 160:251-258 |
| ISSN: | 1423-0097 1018-2438 |
| DOI: | 10.1159/000341642 |
| Popis: | Background: The moderately homologous (approx. 60%) proteins Ara h 2 and Ara h 6 are the most potent peanut allergens. This study was designed to define the relative individual contributions of Ara h 2 and Ara h 6 to the overall allergenic activity of a crude peanut extract (CPE). Methods: Ara h 2 and Ara h 6 were removed from CPE by gel filtration chromatography. Ara h 2.01, Ara h 2.02 and Ara h 6 were further purified (>99%). The potency of each allergen and the ability of these allergens to reconstitute the allergenic activity of CPE depleted of Ara h 2 and Ara h 6 was measured with RBL SX-38 cells sensitized with IgE from sensitized peanut allergic patients. Results: The potency of the native proteins were significantly different (p < 0.0001) although not dramatically so, with a rank order of Ara h 2.01 > Ara h 2.02 > Ara h 6. The addition of either purified Ara h 2 or Ara h 6 independently at their original concentration to CPE depleted of both Ara h 2 and Ara h 6 restored 80–100% of the original CPE allergenic activity. Addition of both Ara h 2 and Ara h 6 consistently completely restored the allergenic activity of CPE. Conclusions: These studies indicate that either Ara h 2 or Ara h 6 independently can account for most of the allergenic activity in a CPE and demonstrate important redundancy in the allergenic activity of these related molecules. |
| Databáze: | OpenAIRE |
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