Database mining and transcriptional analysis of genes encoding inulin-modifying enzymes of Aspergillus niger

Autor: Yuan, X.L., Goosen, C., Kools, H., van der Maare, M.J.E.C., van den Hondel, C.A.M.J., Dijkhuizen, L., Ram, A.F.J., Maarel, Marc J.E.C. van der
Přispěvatelé: Host-Microbe Interactions, Faculty of Science and Engineering, Groningen Biomolecular Sciences and Biotechnology, Rijksuniversiteit Groningen, Bioproduct Engineering, Engineering and Technology Institute Groningen, Moleculaire Microbiologie
Rok vydání: 2006
Předmět:
Sucrose
Transcription
Genetic
Catabolite repression
invertase
chemistry.chemical_compound
MULTIPLE SEQUENCE ALIGNMENT
Microbiologie
CARBON CATABOLITE REPRESSION
2 ENDOINULINASE GENES
Gene Expression Regulation
Fungal
2 endoinulinase genes
DNA
Fungal
GeneralLiterature_REFERENCE(e.g.
dictionaries
encyclopedias
glossaries)
bacillus-subtilis
EXO-INULINASE
Phylogeny
biology
Fungal genetics
Inulin
Biochemistry
ESCHERICHIA-COLI
ComputingMethodologies_DOCUMENTANDTEXTPROCESSING
multiple sequence alignment
Aspergillus niger
Genome
Fungal
exo-inulinase
Sequence analysis
Molecular Sequence Data
INVERTASE
NUCLEOTIDE-SEQUENCES
Fructose
Protein Sorting Signals
Microbiology
BACILLUS-SUBTILIS
RNA
Messenger
VLAG
MOLECULAR-CLONING
Sequence Homology
Amino Acid
beta-Fructofuranosidase
molecular-cloning
transformation
fungi
Computational Biology
RNA
Fungal
biology.organism_classification
carbon catabolite repression
Blotting
Northern
TRANSFORMATION
Invertase
nucleotide-sequences
chemistry
escherichia-coli
Zdroj: Microbiology 152 (2006) 10
Microbiology, 152(10), 3061-3073
Microbiology-Sgm, 152(10), 3061-3073. MAIK NAUKA/INTERPERIODICA/SPRINGER
ISSN: 1350-0872
Popis: As a soil fungus, Aspergillus niger can metabolize a wide variety of carbon sources, employing sets of enzymes able to degrade plant-derived polysaccharides. In this study the genome sequence of A. niger strain CBS 513.88 was surveyed, to analyse the gene/enzyme network involved in utilization of the plant storage polymer inulin, and of sucrose, the substrate for inulin synthesis in plants. In addition to three known activities, encoded by the genes suc1 (invertase activity; designated sucA), inuE (exo-inulinase activity) and inuA/inuB (endo-inulinase activity), two new putative invertase-like proteins were identified. These two putative proteins lack N-terminal signal sequences and therefore are expected to be intracellular enzymes. One of these two genes, designated sucB, is expressed at a low level, and its expression is up-regulated when A. niger is grown on sucrose- or inulin-containing media. Transcriptional analysis of the genes encoding the sucrose- (sucA) and inulin-hydrolysing enzymes (inuA and inuE) indicated that they are similarly regulated and all strongly induced on sucrose and inulin. Analysis of a ΔcreA mutant strain of A. niger revealed that expression of the extracellular inulinolytic enzymes is under control of the catabolite repressor CreA. Expression of the inulinolytic enzymes was not induced by fructose, not even in the ΔcreA background, indicating that fructose did not act as an inducer. Evidence is provided that sucrose, or a sucrose-derived intermediate, but not fructose, acts as an inducer for the expression of inulinolytic genes in A. niger.
Databáze: OpenAIRE
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