Implication of the oep16-1 mutation in a flu-independent, singlet oxygen-regulated cell death pathway in Arabidopsis thaliana
| Autor: | Stephan Pollmann, Diter von Wettstein, Iga Samol, Claudia Rossig, Christiane Reinbothe, Armin Springer, Frank Buhr, Abder Lahroussi, Steffen Reinbothe |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
0106 biological sciences
Programmed cell death Physiology Biología Mutant Arabidopsis Plant Science Biology 01 natural sciences Ion Channels Transcriptome 03 medical and health sciences chemistry.chemical_compound Protochlorophyllide Arabidopsis thaliana 030304 developmental biology chemistry.chemical_classification 0303 health sciences Reactive oxygen species Cell Death Singlet Oxygen Singlet oxygen Arabidopsis Proteins Translation (biology) Cell Biology General Medicine biology.organism_classification Biochemistry chemistry Mutation 010606 plant biology & botany |
| Zdroj: | Plant And Cell Physiology, ISSN 0032-0781, 2011, Vol. 52, No. 1 Archivo Digital UPM instname |
| ISSN: | 1471-9053 |
| Popis: | Singlet oxygen is a prominent form of reactive oxygen species in higher plants. It is easily formed from molecular oxygen by triplet-triplet interchange with excited porphyrin species. Evidence has been obtained from studies on the flu mutant of Arabidopsis thaliana of a genetically determined cell death pathway that involves differential changes at the transcriptome level. Here we report on a different cell death pathway that can be deduced from the analysis of oep16 mutants of A. thaliana. Pure lines of four independent OEP16-deficient mutants with different cell death properties were isolated. Two of the mutants overproduced free protochlorophyllide (Pchlide) in the dark because of defects in import of NADPH:Pchlide oxidoreductase A (pPORA) and died after illumination. The other two mutants avoided excess Pchlide accumulation. Using pulse labeling and polysome profiling studies we show that translation is a major site of cell death regulation in flu and oep16 plants. flu plants respond to photooxidative stress triggered by singlet oxygen by reprogramming their translation toward synthesis of key enzymes involved in jasmonic acid synthesis and stress proteins. In contrast, those oep16 mutants that were prone to photooxidative damage were unable to respond in this way. Together, our results show that translation is differentially affected in the flu and oep16 mutants in response to singlet oxygen. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|