Inhibitory effect of apoptosis in human astrocytes CCF-STTG1 cells by lemon oil
| Autor: | Seung-Heon Hong, Jong-Woong Ahn, Yeung-Su Lyu, Hyung-Min Kim, Yong-Geun Lee, Hyun-Na Koo, Jae-Ju Kim, Chae-Yun Kim |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Citrus
Programmed cell death Hot Temperature Poly ADP ribose polymerase Blotting Western Apoptosis Caspase 3 DNA Fragmentation Cell Line law.invention law Animals Humans Plant Oils Fragmentation (cell biology) Essential oil Caspase Cell Nucleus Electrophoresis Agar Gel Pharmacology biology Brain DNA Flow Cytometry Molecular biology Chromatin Rats Cell biology Enzyme Activation Astrocytes Caspases Depression Chemical biology.protein DNA fragmentation Poly(ADP-ribose) Polymerases |
| Zdroj: | Pharmacological Research. 45:469-473 |
| ISSN: | 1043-6618 |
| DOI: | 10.1006/phrs.2002.0986 |
| Popis: | The effects of lemon pure essential oils on the heat shock-induced apoptosis in human astrocytes cell line CCF-STTG1 were examined. In previous studies, heat shock has been reported to induce the apoptosis or programmed cell death through the activation of caspase-3. Treatment of heat shock on CCF-STTG1 cells markedly induced apoptotic cell death as determined by flow cytometry. Interestingly, pre-treatment with lemon pure essential oils on CCF-STTG1 cells inhibited the heat shock-induced apoptosis. Lemon oil also inhibited the heat shock-induced apoptosis in primary cultured rat astrocytes. To determine whether lemon oil inhibits the heat shock-induced activation of the apoptotic proteases, activation of caspase-3 was assessed by Western blotting. DNA fragmentation, giemsa staining, and heat shock-induced activation of caspase-3 were blocked by lemon pure essential oil, which is consistent with flow cytometry. Poly-ADP-ribose polymerase (PARP), the cysteine protease substrate, was fragmented as a consequence of apoptosis by heat shock. Lemon oil inhibited the PARP fragmentation. These results suggest that lemon pure essential oils may modulate the apoptosis through the activation of the interleukin-1 β -converting enzyme-like caspases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect