Measurement of cortisol in saliva: A comparison of measurement error within and between international academic-research laboratories
| Autor: | Silvanna Verlezza, Frances R. Chen, Sonia J. Lupien, Helen Findlay, Matt Bristow, Jessica L. Calvi, Claire-Dominique Walker, Christine Heim, Eleanor Brindle, Eric A. Hodges, Sonja Entringer, Heiko Klawitter, Alpana De, Douglas A. Granger, Victoria Brann Benson, Holly M. Rus, Jitske Tiemensma |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Adult
Male Saliva Hydrocortisone lcsh:Medicine Physiology Clinical Chemistry Tests Salivary cortisol Inter-assay General Biochemistry Genetics and Molecular Biology Inter-laboratory variation Immunoenzyme Techniques Young Adult 03 medical and health sciences 0302 clinical medicine Intra-assay Humans Medicine Whole saliva lcsh:Science (General) lcsh:QH301-705.5 Measurement variability business.industry lcsh:R Reproducibility of Results Mean age General Medicine 030227 psychiatry Research Note lcsh:Biology (General) Immunology Female business 030217 neurology & neurosurgery lcsh:Q1-390 |
| Zdroj: | Calvi, JL; Chen, FR; Benson, VB; Brindle, E; Bristow, M; De, A; et al.(2017). Measurement of cortisol in saliva: A comparison of measurement error within and between international academic-research laboratories. BMC Research Notes, 10(1). doi: 10.1186/s13104-017-2805-4. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/5s43f8nw BMC Research Notes, Vol 10, Iss 1, Pp 1-6 (2017) BMC Research Notes |
| Popis: | © 2017 The Author(s). Objective: Hundreds of scientific publications are produced annually that involve the measurement of cortisol in saliva. Intra- and inter-laboratory variation in salivary cortisol results has the potential to contribute to cross-study inconsistencies in findings, and the perception that salivary cortisol results are unreliable. This study rigorously estimates sources of measurement variability in the assay of salivary cortisol within and between established international academic-based laboratories that specialize in saliva analyses. One hundred young adults (Mean age: 23.10 years; 62 females) donated 2 mL of whole saliva by passive drool. Each sample was split into multiple- 100 μL aliquots and immediately frozen. One aliquot of each of the 100 participants' saliva was transported to academic laboratories (N = 9) in the United States, Canada, UK, and Germany and assayed for cortisol by the same commercially available immunoassay. Results: 1.76% of the variance in salivary cortisol levels was attributable to differences between duplicate assays of the same sample within laboratories, 7.93% of the variance was associated with differences between laboratories, and 90.31% to differences between samples. In established-qualified laboratories, measurement error of salivary cortisol is minimal, and inter-laboratory differences in measurement are unlikely to have a major influence on the determined values. |
| Databáze: | OpenAIRE |
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