Bioinformatics Analyses Indicate That Cathepsin G (CTSG) is a Potential Immune-Related Biomarker in Oral Squamous Cell Carcinoma (OSCC)
Autor: | Yu-Xing Song, Fei Li, Guang-Zhao Huang, Qing-qing Wu, Xinyan Lu, Wei-Sen Zeng, Xiaozhi Lv, Heng-Yu Ye, Ze-nan Zheng, Gao-Xiang Chen, Yi-Long Ai, Ting-ru Shao |
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Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Cathepsin G Biology OncoTargets and Therapy 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Immune system Gene expression medicine Pharmacology (medical) Gene IRGs Survival analysis Original Research Cancer bioinformatics medicine.disease oral squamous cell carcinoma stomatognathic diseases 030104 developmental biology Oncology chemistry 030220 oncology & carcinogenesis Cancer research biomarker Biomarker (medicine) immune CTSG |
Zdroj: | OncoTargets and therapy |
ISSN: | 1178-6930 |
Popis: | Guang-zhao Huang,1,* Qing-qing Wu,1,* Ze-nan Zheng,1,* Ting-ru Shao,1 Fei Li,1 Xin-yan Lu,1 Heng-yu Ye,1 Gao-xiang Chen,1 Yu-xing Song,1 Wei-sen Zeng,2 Yi-long Ai,3 Xiao-zhi Lv1 1Department of Oral & Maxillofacial Surgery, NanFang Hospital, Southern Medical University, Guangzhou, Guangdong Province, People’s Republic of China; 2Department of Cell Biology, School of Basic Medical Science, Southern Medical University, Guangzhou, Guangdong Province, People’s Republic of China; 3Foshan Stomatological Hospital, School of Stomatology and Medicine, Foshan University, Foshan, Guangdong Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiao-zhi LvDepartment of Oral & Maxillofacial Surgery, NanFang Hospital, Southern Medical University, Baiyun District, Guangzhou, Guangdong Province, 510080, People’s Republic of ChinaTel +86 13724811658Email lxzsurgeon@126.comYi-long AiFoshan Stomatological Hospital, School of Stomatology and Medicine, Foshan University, Chancheng District, Foshan, Guangdong Province, 528000, People’s Republic of ChinaTel +86 13827755774Email aiyilong@126.comPurpose: Plenty of studies showed that the immune system was associated with cancer initiation and progression. This study aimed to explore the prognostic biomarkers from immune-related genes (IRGs) in oral squamous cell carcinoma (OSCC).Materials and Methods: RNA-seq data were downloaded from The Cancer Genome Atlas (TCGA) and IRGs and transcription factors (TFs) were extracted. Then, the co-expression network between IRGs and TFs was constructed using the “WGCNA” package in R software. Furthermore, a gene expression signature according to IRGs was constructed to predict OSCC prognosis and its accuracy was validated by survival analysis. Subsequently, correlation analyses between risk-score and immune cells level and clinical parameters were performed. Finally, immune-related biomarkers were selected and further investigated using gain-of-function assays in vitro.Results: A total of 32 normal cases and 317 OSCC cases were selected in our study. Differentially-expressed analysis indicated that there were 381 differentially-expressed IRGs and 62 TFs in OSCC. Among them, 25 TFs and 21 IRGs were enrolled in the co-expression network. Furthermore, we found that gene expression signature on the basis of 10 IRGs could predict the prognosis accurately and a high-risk score based on gene expression signature meant a high T classification, terminal clinical stage, and low immune cells level in OSCC. Finally, cathepsin G (CTSG) was identified as a potential immune-related biomarker and therapeutic target in OSCC.Conclusion: In conclusion, IRGs were directly involved in the development and progression of OSCC. Furthermore, CTSG was identified as a potential independent biomarker and might be an immunotherapeutic target in OSCC treatment.Keywords: oral squamous cell carcinoma, immune, biomarker, bioinformatics, CTSG |
Databáze: | OpenAIRE |
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