Development and validation of RP-HPLC assay of chlorhexidine in gingival crevicular fluid
| Autor: | Ugrinova Liljana, Bogdanovska Liljana, Petkovska Rumenka, Popovska Mirjana, Dimitrovska Aneta, Saliu Sehmedin |
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| Rok vydání: | 2014 |
| Předmět: |
Pharmacology
Chromatography Materials science Chlorhexidine Phosphate buffered saline rp-hplc periodontal disease lcsh:RS1-441 Pharmaceutical Science Chronic periodontal disease High-performance liquid chromatography lcsh:Pharmacy and materia medica Crevicular fluid chemistry.chemical_compound Chromatographic separation gingival crevicular fluid chemistry medicine chlorhexidine digluconate Acetonitrile Triethylamine periochiptm medicine.drug |
| Zdroj: | Arhiv za farmaciju, Vol 64, Iss 2, Pp 69-82 (2014) |
| ISSN: | 0004-1963 |
| Popis: | A reversed - phase HPLC method with UV detection for determination of chlorhexidine in gingival crevicular fluid (GCF) was optimized and validated, using chlorpheniramine as an internal standard. The chromatographic separation was performed on Discovery C18 HPLC column with 0.01 mol L-1 phosphate buffer (pH=3.0), triethylamine and acetonitrile (66:1:33, V/V/V), as mobile phase. Under the optimized HPLC conditions, linearity was obtained in the range of 0.5-5.0 μg mL-1 with LOD 0.07 μg mL-1 and LLOQ 0.5 μg mL-1. The described method can be successfully applied for determination of chlorhexidine concentrations in GCF obtained from patients with chronic periodontal disease treated with PerioChipTM. |
| Databáze: | OpenAIRE |
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